Gomez, Ingrid Ward, Ben Souilhol, Celine Recarti, Chiara Ariaans, Mark Johnston, Jessica Burnett, Amanda Mahmoud, Marwa Le Anh Luong West, Laura Long, Merete Parry, Sion Woods, Rachel Hulston, Carl Benedikter, Birke Niespolo, Chiara Bazaz, Rohit Francis, Sheila Kiss-Toth, Endre van Zandvoort, Marc Schober, Andreas Hellewell, Paul Evans, Paul C Ridger, Victoria Neutrophil microvesicles drive atherosclerosis by delivering <i>miR-155</i> to atheroprone endothelium Neutrophils are implicated in the pathogenesis of atherosclerosis but are seldom detected in atherosclerotic plaques. We investigated whether neutrophil-derived microvesicles may influence arterial pathophysiology. Here we report that levels of circulating neutrophil microvesicles are enhanced by exposure to a high fat diet, a known risk factor for atherosclerosis. Neutrophil microvesicles accumulate at disease-prone regions of arteries exposed to disturbed flow patterns, and promote vascular inflammation and atherosclerosis in a murine model. Using cultured endothelial cells exposed to disturbed flow, we demonstrate that neutrophil microvesicles promote inflammatory gene expression by delivering miR-155, enhancing NF-κB activation. Similarly, neutrophil microvesicles increase miR-155 and enhance NF-κB at disease-prone sites of disturbed flow in vivo. Enhancement of atherosclerotic plaque formation and increase in macrophage content by neutrophil microvesicles is dependent on miR-155. We conclude that neutrophils contribute to vascular inflammation and atherogenesis through delivery of microvesicles carrying miR-155 to disease-prone regions. Atherosclerosis;Lymphocytes;miRNAs 2020-01-24
    https://repository.lboro.ac.uk/articles/journal_contribution/Neutrophil_microvesicles_drive_atherosclerosis_by_delivering_i_miR-155_i_to_atheroprone_endothelium/11695362