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3D bioprinting – Flow cytometry as analytical strategy for 3D cell structures

journal contribution
posted on 2019-04-08, 14:08 authored by Sarah Gretzinger, Nicole Beckert, Andy GleadallAndy Gleadall, Cornelia Lee-Thedieck, Jürgen Hubbuch
The importance of 3D printing technologies increased significantly over the recent years. They are considered to have a huge impact in regenerative medicine and tissue engineering, since 3D bioprinting enables the production of cell-laden 3D scaffolds. Transition from academic research to pharmaceutical industry or clinical applications, however, is highly dependent on developing a robust and well-known process, while maintaining critical cell characteristics. Hence, a directed and systematic approach to 3D bioprinting process development is required, which also allows for the monitoring of these cell characteristics. This work presents the development of a flow cytometry-based analytical strategy as a tool for 3D bioprinting research. The development was based on a model process using a commercially available alginate-based bioink, the β-cell line INS-1E, and direct dispensing as 3D bioprinting method. We demonstrated that this set-up enabled viability and proliferation analysis. Additionally, use of an automated sampler facilitated high-throughput screenings. Finally, we showed that each process step, e.g. suspension of cells in bioink or 3D printing, cross-linking of the alginate scaffold after printing, has a crucial impact on INS-1E viability. This reflects the importance of process optimization in 3D bioprinting and the usefulness of the flow cytometry-based analytical strategy described here. The presented strategy has a great potential as a cell characterisation tool for 3D bioprinting and may contribute to a more directed process development.

Funding

This work was supported by the Helmholtz Program “BioInterfaces in Technology and Medicine (BIFTM).”

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Bioprinting

Volume

11

Pages

e00023 - e00023

Citation

GRETZINGER, S. ... et al, 2018. 3D bioprinting – Flow cytometry as analytical strategy for 3D cell structures. Bioprinting, 11, e00023.

Publisher

© Elsevier BV

Version

  • VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Acceptance date

2018-04-09

Publication date

2018

Notes

This paper is closed access.

ISSN

2405-8866

Language

  • en

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