File(s) under permanent embargo
Reason: This item is currently closed access.
3D bioprinting – Flow cytometry as analytical strategy for 3D cell structures
journal contribution
posted on 2019-04-08, 14:08 authored by Sarah Gretzinger, Nicole Beckert, Andy GleadallAndy Gleadall, Cornelia Lee-Thedieck, Jürgen HubbuchThe importance of 3D printing technologies increased significantly over the recent years. They are considered to have a huge impact in regenerative medicine and tissue engineering, since 3D bioprinting enables the production of cell-laden 3D scaffolds. Transition from academic research to pharmaceutical industry or clinical applications, however, is highly dependent on developing a robust and well-known process, while maintaining critical cell characteristics. Hence, a directed and systematic approach to 3D bioprinting process development is required, which also allows for the monitoring of these cell characteristics. This work presents the development of a flow cytometry-based analytical strategy as a tool for 3D bioprinting research. The development was based on a model process using a commercially available alginate-based bioink, the β-cell line INS-1E, and direct dispensing as 3D bioprinting method. We demonstrated that this set-up enabled viability and proliferation analysis. Additionally, use of an automated sampler facilitated high-throughput screenings. Finally, we showed that each process step, e.g. suspension of cells in bioink or 3D printing, cross-linking of the alginate scaffold after printing, has a crucial impact on INS-1E viability. This reflects the importance of process optimization in 3D bioprinting and the usefulness of the flow cytometry-based analytical strategy described here. The presented strategy has a great potential as a cell characterisation tool for 3D bioprinting and may contribute to a more directed process development.
Funding
This work was supported by the Helmholtz Program “BioInterfaces in Technology and Medicine (BIFTM).”
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
BioprintingVolume
11Pages
e00023 - e00023Citation
GRETZINGER, S. ... et al, 2018. 3D bioprinting – Flow cytometry as analytical strategy for 3D cell structures. Bioprinting, 11, e00023.Publisher
© Elsevier BVVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Acceptance date
2018-04-09Publication date
2018Notes
This paper is closed access.ISSN
2405-8866Publisher version
Language
- en