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Tao Sun
Tao
Sun
Yiping Liu
Yiping
Liu
Fengrong Bu
Fengrong
Bu
Ge Chen
Ge
Chen
Meijuan Wen
Meijuan
Wen
Purification of prothrombin in Nitschmann fraction III by membrane radial column ion-exchange liquid chromatograph
Loughborough University
2017
Membrane radial column chromatography
Nitschmann fraction Ⅲ
Prothrombin complex concentrate
Purification
Chemical Engineering not elsewhere classified
2017-05-12 15:00:32
Journal contribution
https://repository.lboro.ac.uk/articles/journal_contribution/Purification_of_prothrombin_in_Nitschmann_fraction_III_by_membrane_radial_column_ion-exchange_liquid_chromatograph/9241001
method is described for the purification of human prothrombin complex concentrate ( PCC) from
Nitschmann fraction Ⅲ by membrane radial column ion2exchange chromatography , which allows large sample
volumes to be processed at low operation pressure. The Nitschmann fraction Ⅲ(15 g) was mixed with 1 000 mL 0.
06 mol/ L Tris2HCl (pH 7. 5) . The centrifuged supernatant (10 000 r/ min ,15 min ,20 ℃) was applied onto a DEAE
ion2exchange liquid chromatographic column (XK216 DEAE fast flow Sepharose and DEAE membrane radical column
chromatography) with almost the same excellent separation efficiency. The parameters of sample flow rate , elution
flow rate and sample capacity were optimized.