2134/24983 Tao Sun Tao Sun Yiping Liu Yiping Liu Fengrong Bu Fengrong Bu Ge Chen Ge Chen Meijuan Wen Meijuan Wen Purification of prothrombin in Nitschmann fraction III by membrane radial column ion-exchange liquid chromatograph Loughborough University 2017 Membrane radial column chromatography Nitschmann fraction Ⅲ Prothrombin complex concentrate Purification Chemical Engineering not elsewhere classified 2017-05-12 15:00:32 Journal contribution https://repository.lboro.ac.uk/articles/journal_contribution/Purification_of_prothrombin_in_Nitschmann_fraction_III_by_membrane_radial_column_ion-exchange_liquid_chromatograph/9241001 method is described for the purification of human prothrombin complex concentrate ( PCC) from Nitschmann fraction Ⅲ by membrane radial column ion2exchange chromatography , which allows large sample volumes to be processed at low operation pressure. The Nitschmann fraction Ⅲ(15 g) was mixed with 1 000 mL 0. 06 mol/ L Tris2HCl (pH 7. 5) . The centrifuged supernatant (10 000 r/ min ,15 min ,20 ℃) was applied onto a DEAE ion2exchange liquid chromatographic column (XK216 DEAE fast flow Sepharose and DEAE membrane radical column chromatography) with almost the same excellent separation efficiency. The parameters of sample flow rate , elution flow rate and sample capacity were optimized.