Thomas, Rob Ratcliffe, Elizabeth Automated adherent human cell culture (mesenchymal stem cells) Human cell culture processes developed at research laboratory scale need to be translated to large-scale production processes to achieve commercial application to a large market. To allow this transition of scale with consistent process performance and control of costs, it will be necessary to reduce manual processing and increase automation. There are a number of commercially available platforms that will reduce manual process intervention and improve process control for different culture formats. However, in many human cell-based applications, there is currently a need to remain close to the development format, usually adherent culture on cell culture plastic or matrix-coated wells or flasks due to deterioration of cell quality in other environments, such as suspension. This chapter presents an example method for adherent automated human stem cell culture using a specific automated flask handling platform, the CompacT SelecT. Alkaline phosphatase;Automation;Flow cytometry;Humans;Mesenchymal stem cells;Osteogenesis;Stem cells;Chemical Engineering not elsewhere classified 2017-08-21
    https://repository.lboro.ac.uk/articles/journal_contribution/Automated_adherent_human_cell_culture_mesenchymal_stem_cells_/9241133