2134/16628 Atul Nayak Atul Nayak Diganta Das Diganta Das Goran Vladisavljevic Goran Vladisavljevic Microneedle-assisted permeation of lidocaine carboxymethylcellulose with gelatine co-polymer hydrogel Loughborough University 2015 Gelatine Hydrogel In vitro skin permeation Lidocaine Microneedles Sodium carboxymethylcellulose Chemical Engineering not elsewhere classified 2015-01-23 14:45:10 Journal contribution https://repository.lboro.ac.uk/articles/journal_contribution/Microneedle-assisted_permeation_of_lidocaine_carboxymethylcellulose_with_gelatine_co-polymer_hydrogel/9243116 Purpose Lidocaine hydrochloride (LidH) was formulated in sodium carboxymethyl cellulose/ gelatine (NaCMC/GEL) hydrogel and a ‘poke and patch’ microneedle delivery method was used to enhance permeation flux of LidH. Methods The microparticles were formed by electrostatic interactions between NaCMC and GEL macromolecules within a water/oil emulsion in paraffin oil and the covalent crosslinking was by glutaraldehyde. The GEL to NaCMC mass ratio was varied between 1.6 and 2.7. The LidH encapsulation yield was 1.2 to 7% w/w. LidH NaCMC/GEL was assessed for encapsulation efficiency, zeta potential, mean particle size and morphology. Subsequent in vitro skin permeation studies were performed via passive diffusion and microneedle assisted permeation of LidH NaCMC/GEL to determine the maximum permeation rate through full thickness skin. Results LidH 2.4% w/w NaCMC/GEL 1:1.6 and 1:2.3 respectively, possessed optimum zeta potential. LidH 2.4% w/ w NaCMC/GEL 1:2.3 and 1:2.7 demonstrate higher pseudoplastic behaviour. Encapsulation efficiency (14.9–17.2%) was similar for LidH 2.4% w/w NaCMC/GEL 1:1.6–1:2.3. Microneedle assisted permeation flux was optimum for LidH 2.4% w/w NaCMC/GEL 1:2.3 at 6.1 μg/ml/h. Conclusion LidH 2.4% w/w LidH NaCMC/GEL 1:2.3 crossed the minimum therapeutic drug threshold with microneedle skin permeation in less than 70 min.