Barbosa, Ana I. Gehlot, Poonam Sidapra, Kalpita Edwards, Alexander D. Reis, Nuno Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device We present a new, power-free and flexible detection system named MCFphone for portable colorimetric and fluorescence quantitative sandwich immunoassay detection of prostate specific antigen (PSA). The MCFphone is composed by a smartphone integrated with a magnifying lens, a simple light source and a miniaturised immunoassay platform, the Microcapillary Film (MCF). The excellent transparency and flat geometry of fluoropolymer MCF allowed quantitation of PSA in the range 0.9 to 60. ng/ml with<7% precision in 13. min using enzymatic amplification and a chromogenic substrate. The lower limit of detection was further improved from 0.4 to 0.08. ng/ml in whole blood samples with the use of a fluorescence substrate. The MCFphone has shown capable of performing rapid (13 to 22. min total assay time) colorimetric quantitative and highly sensitive fluorescence tests with good %Recovery, which represents a major step in the integration of a new generation of inexpensive and portable microfluidic devices with commercial immunoassay reagents and off-the-shelf smartphone technology. Colorimetric;Fluorescence;Microfluidic;Miniaturised ELISA;Point of care diagnostics;Smartphone readout;Chemical Engineering not elsewhere classified 2015-04-22
    https://repository.lboro.ac.uk/articles/journal_contribution/Portable_smartphone_quantitation_of_prostate_specific_antigen_PSA_in_a_fluoropolymer_microfluidic_device/9244460