%0 Journal Article %A Managh, Amy %A Edwards, Sheldon L. %A Bushell, Andrew %A Wood, Kathryn J. %A Geissler, Edward K. %A Hutchinson, James A. %A Hutchinson, Robert W. %A Reid, Helen %A Sharp, Barry %D 2014 %T Single cell tracking of gadolinium labeled CD4(+) T cells by laser ablation inductively coupled plasma mass spectrometry %U https://repository.lboro.ac.uk/articles/journal_contribution/Single_cell_tracking_of_gadolinium_labeled_CD4_T_cells_by_laser_ablation_inductively_coupled_plasma_mass_spectrometry/9391004 %2 https://repository.lboro.ac.uk/ndownloader/files/17004437 %K untagged %K Chemical Sciences not elsewhere classified %X Cellular therapy is emerging as a promising alternative to conventional immunosuppression in the fields of haematopoietic stem cell (HSC) transplantation, autoimmune disease and solid organ transplantation. Determining the persistence of cell-based therapies in vivo is crucial to understanding their regulatory function and requires the combination of an extremely sensitive detection technique and a stable, long-lifetime cell labelling agent. This paper reports the first application of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to perform single cell detection of T cell populations relevant to cellular immunotherapy. Purified human CD4+ T cells were labelled with commercially available Gd-based MRI contrast agents, OmniscanĀ® and DotaremĀ®, which enabled passive loading of up to 108 Gd atoms per cell. In mixed preparations of labelled and unlabelled cells, LA-ICP-MS was capable of enumerating labelled cells at close to the predicted ratio. More importantly, LA-ICP-MS single cell analysis demonstrated that the cells retained sufficient label to remain detectable for up to 10 days post-labelling both in vitro and in vivo in an immunodeficient mouse model. %I Loughborough University