2134/36611
Benita C. Percival
Benita C.
Percival
Martin Grootveld
Martin
Grootveld
Miles Gibson
Miles
Gibson
Yasan Osman
Yasan
Osman
Marco Molinari
Marco
Molinari
Fereshteh Jafari
Fereshteh
Jafari
Tarsem Sahota
Tarsem
Sahota
Mark Martin
Mark
Martin
Federico Casanova
Federico
Casanova
Melissa L. Mather
Melissa L.
Mather
Mark Edgar
Mark
Edgar
Jinit Masania
Jinit
Masania
Philippe B. Wilson
Philippe B.
Wilson
Low-field, benchtop NMR spectroscopy as a potential tool for point-of-care diagnostics of metabolic conditions: Validation, protocols and computational models
Loughborough University
2019
Metabolomics
Benchtop 60 MHz NMR analysis
Biomarkers
Biomolecules
Validation
Protocol
Diabetes
Chemical Sciences not elsewhere classified
2019-01-17 11:41:23
Journal contribution
https://repository.lboro.ac.uk/articles/journal_contribution/Low-field_benchtop_NMR_spectroscopy_as_a_potential_tool_for_point-of-care_diagnostics_of_metabolic_conditions_Validation_protocols_and_computational_models/9392414
Novel sensing technologies for liquid biopsies offer promising prospects for the early detection of metabolic conditions through omics techniques. Indeed, high-field nuclear magnetic resonance (NMR) facilities are routinely used for metabolomics investigations on a range of biofluids in order to rapidly recognise unusual metabolic patterns in patients suffering from a range of diseases. However, these techniques are restricted by the prohibitively large size and cost of such facilities, suggesting a possible role for smaller, low-field NMR instruments in biofluid analysis. Herein we describe selected biomolecule validation on a low-field benchtop NMR spectrometer (60 MHz), and present an associated protocol for the analysis of biofluids on compact NMR instruments. We successfully detect common markers of diabetic control at low-to-medium concentrations through optimised experiments, including α-glucose (≤2.8 mmol/L) and acetone (25 µmol/L), and additionally in readily accessible biofluids, particularly human urine. We present a combined protocol for the analysis of these biofluids with low-field NMR spectrometers for metabolomics applications, and offer a perspective on the future of this technique appealing to ‘point-of-care’ applications.