2134/36611 Benita C. Percival Benita C. Percival Martin Grootveld Martin Grootveld Miles Gibson Miles Gibson Yasan Osman Yasan Osman Marco Molinari Marco Molinari Fereshteh Jafari Fereshteh Jafari Tarsem Sahota Tarsem Sahota Mark Martin Mark Martin Federico Casanova Federico Casanova Melissa L. Mather Melissa L. Mather Mark Edgar Mark Edgar Jinit Masania Jinit Masania Philippe B. Wilson Philippe B. Wilson Low-field, benchtop NMR spectroscopy as a potential tool for point-of-care diagnostics of metabolic conditions: Validation, protocols and computational models Loughborough University 2019 Metabolomics Benchtop 60 MHz NMR analysis Biomarkers Biomolecules Validation Protocol Diabetes Chemical Sciences not elsewhere classified 2019-01-17 11:41:23 Journal contribution https://repository.lboro.ac.uk/articles/journal_contribution/Low-field_benchtop_NMR_spectroscopy_as_a_potential_tool_for_point-of-care_diagnostics_of_metabolic_conditions_Validation_protocols_and_computational_models/9392414 Novel sensing technologies for liquid biopsies offer promising prospects for the early detection of metabolic conditions through omics techniques. Indeed, high-field nuclear magnetic resonance (NMR) facilities are routinely used for metabolomics investigations on a range of biofluids in order to rapidly recognise unusual metabolic patterns in patients suffering from a range of diseases. However, these techniques are restricted by the prohibitively large size and cost of such facilities, suggesting a possible role for smaller, low-field NMR instruments in biofluid analysis. Herein we describe selected biomolecule validation on a low-field benchtop NMR spectrometer (60 MHz), and present an associated protocol for the analysis of biofluids on compact NMR instruments. We successfully detect common markers of diabetic control at low-to-medium concentrations through optimised experiments, including α-glucose (≤2.8 mmol/L) and acetone (25 µmol/L), and additionally in readily accessible biofluids, particularly human urine. We present a combined protocol for the analysis of these biofluids with low-field NMR spectrometers for metabolomics applications, and offer a perspective on the future of this technique appealing to ‘point-of-care’ applications.