2134/33947
Guangle Niu
Guangle
Niu
Ruoyao Zhang
Ruoyao
Zhang
John P. Kwong
John P.
Kwong
Jacky W.Y. Lam
Jacky W.Y.
Lam
Congping Chen
Congping
Chen
Jianguo Wang
Jianguo
Wang
Yuncong Chen
Yuncong
Chen
Xing Feng
Xing
Feng
Ryan T. Kwok
Ryan T.
Kwok
Herman H.-Y. Sung
Herman H.-Y.
Sung
Ian D. Williams
Ian D.
Williams
Mark Elsegood
Mark
Elsegood
Jianan Qu
Jianan
Qu
Chao Ma
Chao
Ma
Kam S. Wong
Kam S.
Wong
Xiaoqiang Yu
Xiaoqiang
Yu
Ben Zhong Tang
Ben Zhong
Tang
Specific two-photon imaging of live cellular and deep-tissue lipid droplets by lipophilic AIEgens at ultra-low concentration
Loughborough University
2018
untagged
Chemical Sciences not elsewhere classified
2018-07-17 09:58:47
Journal contribution
https://repository.lboro.ac.uk/articles/journal_contribution/Specific_two-photon_imaging_of_live_cellular_and_deep-tissue_lipid_droplets_by_lipophilic_AIEgens_at_ultra-low_concentration/9393311
Lipid droplets are highly associated with obesity, diabetes, inflammatory disorders and cancer. A reliable two-photon dye for specific lipid droplets imaging in live cells and live tissues at ultra-low concentration has rarely been reported. In this work, four new aggregation-induced emission luminogens (AIEgens) based on the naphthalene core
were designed and synthesized for specific two-photon lipid droplets staining. The new molecules, namely NAP AIEgens, exhibit large Stokes shift (>110 nm), high solid-state fluorescence quantum yield (up to 30%), good two-photon absorption cross section (45–100 GM at 860 nm), high biocompatibility and good photostability. They could specifically stain lipid droplets at ultra-low concentration (50 nM) in a short time of 15 min. Such ultra-low concentration is the lowest value for lipid droplets staining in live cells reported so far. In vitro and ex vivo two-photon imaging of lipid droplets in live cells and live mice liver tissues were successfully demonstrated. In addition, selective visualization of lipid droplets in live mice liver tissues could be achieved at a depth of about 70 μm. These excellent properties render them as promising candidates for investigating lipid droplets-associated physiological and pathological processes in live biological samples.