%0 Journal Article %A Devenport, Neil A. %A Reynolds, Jim %A Parkash, Ved %A Cook, Jason %A Weston, Daniel J. %A Creaser, Colin %D 2011 %T Determination of free desmosine and isodesmosine as urinary biomarkers of lung disorder by ultra performance liquid chromatography-ion mobility-mass spectrometry %U https://repository.lboro.ac.uk/articles/journal_contribution/Determination_of_free_desmosine_and_isodesmosine_as_urinary_biomarkers_of_lung_disorder_by_ultra_performance_liquid_chromatography-ion_mobility-mass_spectrometry/9393842 %2 https://repository.lboro.ac.uk/ndownloader/files/17008166 %K Ion mobility-mass spectrometry %K Urinary analysis %K COPD %K Desmosine %K Isodesmosine %K Chemical Sciences not elsewhere classified %X The elastin degradation products, desmosine (DES) and isodesmosine (IDES) are highly stable, cross-linking amino-acids that are unique to mature elastin. The excretion of DES/IDES in urine, in the free form and with associated peptide fragments, provides an indicator of lung damage in chronic obstructive pulmonary disease (COPD). A quantitative ion mobility-mass spectrometry (IM-MS) method has been developed for the analysis of free DES/IDES in urine with deuterated IDES as an internal standard. Resolution of DES/IDES isomers was achieved in less than five minutes using ultra performance liquid chromatography (UPLC) combined with ion pairing. The optimized UPLC-IM-MS method provided a linear dynamic range of 10-300 ng/mL and a limit of quantitation of 0.028 ng/mL for IDES and 0.03 ng/mL for DES (0.55 ng and 0.61 ng on column respectively). The method reproducibility (%RSD) was < 4% for DES and IDES. The UPLC-IM-MS method was applied to the analysis of urine samples obtained from healthy volunteers and COPD patients. The DES/IDES concentrations in healthy and COPD urine showed an increase in DES (79%) and IDES (74%) in the COPD samples, relative to healthy controls. The incorporation of an IM separation prior to m/z measurement by MS was shown to reduce non-target ion responses from the bio-fluid matrix. %I Loughborough University