%0 Journal Article %A Reis, Nuno %A Castanheira, Ana P. %A Barbosa, Ana I. %A Edwards, Alexander D. %D 2015 %T Multiplexed femtomolar quantitation of human cytokines in a fluoropolymer microcapillary film %U https://repository.lboro.ac.uk/articles/journal_contribution/Multiplexed_femtomolar_quantitation_of_human_cytokines_in_a_fluoropolymer_microcapillary_film/9394478 %2 https://repository.lboro.ac.uk/ndownloader/files/17008997 %K untagged %K Chemical Sciences not elsewhere classified %X Sensitive quantitation of multiple cytokines can provide important diagnostic information during infection, inflammation and immunopathology. In this study sensitive immunoassay detection of human cytokines IL-1β, IL-6, IL-12p70 and TNFα is shown for singleplex and multiplex formats using a novel miniaturized ELISA platform. The platform uses a disposable plastic multi-syringe aspirator (MSA) integrating 8 disposable fluoropolymer microfluidic test strips, each containing an array of ten 200 μm mean i.d. microcapillaries coated with a set of monoclonal antibodies. Each MSA device thus performs 10 tests on 8 samples, delivering 80 measurements. Unprecedented levels of sensitivity were obtained with the novel fluoropolymer microfluidic material and simple colorimetric detection in a flatbed scanner. The limit of detection for singleplex detection ranged from 2.0 to 15.0 pg/ml, i.e. 35 and 713 femtomolar for singleplex cytokine detection, and the intra- and inter-assay coefficient of variation (CV) remained within 10%. In addition, a triplex immunoassay was developed for measuring IL-1β, IL-12p70 and TNFα simultaneously from a given sample in the pg/ml range. These assays permit high sensitivity measurement with rapid <15 min assay or detection from undiluted blood serum. The portability, speed and low-cost of this system are highly suited to point-of-care testing and field diagnostics applications. %I Loughborough University