2134/18774
Stephen Butler
Stephen
Butler
Ratiometric detection of adenosine triphosphate (ATP) in water and real-time monitoring of apyrase activity with a tripodal zinc complex
Loughborough University
2015
Anions
ATP
Enzymes
Fluorescent probes
Quinolines
Chemical Sciences not elsewhere classified
2015-09-15 15:16:10
Journal contribution
https://repository.lboro.ac.uk/articles/journal_contribution/Ratiometric_detection_of_adenosine_triphosphate_ATP_in_water_and_real-time_monitoring_of_apyrase_activity_with_a_tripodal_zinc_complex/9394787
Two tripodal fluorescent probes Zn⋅L1,2 have been synthesised, and their anion-binding capabilities were examined by using fluorescence spectroscopy. Probe Zn⋅L1 allows the selective and ratiometric detection of adenosine triphosphate (ATP) at physiological pH, even in the presence of several competing anions, such as ADP, phosphate and bicarbonate. The probe was applied to the real-time monitoring of the apyrase-catalysed hydrolysis of ATP, in a medium that mimics an extracellular fluid.