2134/18774 Stephen Butler Stephen Butler Ratiometric detection of adenosine triphosphate (ATP) in water and real-time monitoring of apyrase activity with a tripodal zinc complex Loughborough University 2015 Anions ATP Enzymes Fluorescent probes Quinolines Chemical Sciences not elsewhere classified 2015-09-15 15:16:10 Journal contribution https://repository.lboro.ac.uk/articles/journal_contribution/Ratiometric_detection_of_adenosine_triphosphate_ATP_in_water_and_real-time_monitoring_of_apyrase_activity_with_a_tripodal_zinc_complex/9394787 Two tripodal fluorescent probes Zn⋅L1,2 have been synthesised, and their anion-binding capabilities were examined by using fluorescence spectroscopy. Probe Zn⋅L1 allows the selective and ratiometric detection of adenosine triphosphate (ATP) at physiological pH, even in the presence of several competing anions, such as ADP, phosphate and bicarbonate. The probe was applied to the real-time monitoring of the apyrase-catalysed hydrolysis of ATP, in a medium that mimics an extracellular fluid.