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Hassan M. Albishri
Hassan M.
Albishri
High-temperature HPLC of pharmaceutical compounds
Loughborough University
2018
untagged
Chemical Sciences not elsewhere classified
2018-11-21 10:04:22
Thesis
https://repository.lboro.ac.uk/articles/thesis/High-temperature_HPLC_of_pharmaceutical_compounds/9397817
In this thesis, we established methods that use high-temperature HPLC for the
efficient separation of different groups of pharmaceutical compounds. These groups
are cephalosporins, non-steroidal anti-inflammatory (NSAID), and diuretic drugs.
The effect of changes of the temperature of the column was studied with respect to
various chromatographic parameters: retention factors, thermodynamic properties,
selectivity, efficiency and peak shape.
Three columns (Pathfinder, Xterra RP18, and Selerity's Blaze 200) were examined
for the analysis of cephalosporin drugs under high temperature reversed phase
HPLC. Several proportions of mobile phases were used with these columns. This
study focused on the effect of column temperature on selectivity (α), and efficiency
(N/m), van't Hoff plots. The best conditions for the separation of these drugs were 60 °C with 100% water as eluent and these conditions were the best for quantitative
applications. This condition was validated according to International Conference on
Harmonisation (ICR) with respects of linearity, limit of detection (LOD), limit of
quantification (LOQ), and precision (tR, relative peak area %, N/m). [Continues.]