%0 Thesis %A Montenegro, Ana M.C. %D 2013 %T Studies of column packings for polymer separations by size exclusion and interactive chromatography %U https://repository.lboro.ac.uk/articles/thesis/Studies_of_column_packings_for_polymer_separations_by_size_exclusion_and_interactive_chromatography/9398306 %2 https://repository.lboro.ac.uk/ndownloader/files/17013812 %K untagged %K Chemical Sciences not elsewhere classified %X The chromatographic behaviour of a new column packing for high performance size exclusion chromatography based on crosslinked polyacrylamide particles was studied. Experimental retention data for poly(ethylene glycol)and poly(ethylene oxide) standards in water and water-methanol mixtures showed that the separation mechanism is size exclusion, since a universal calibration plot based on hydrodynamic volume was obtained. For polysaccharide standards in water and water-methanol (80/20), the universal calibration was valid for molecular weights above 4 * 10~. Below this value, secondary mechanisms appear to be taking part in the separation since water-methanol (80/20) is a poor solvent for polysaccharides. Crosslinked polyacrylamide packings showed an interactive behaviour with tetrahydrofuran and dimethylformamide as eluents with polystyrene and poly( ethylene glycol )/poly(ethylene oxide) standards and the universal calibration was not valid.... %I Loughborough University