2134/4240
Rob Thomas
Rob
Thomas
David Anderson
David
Anderson
Amit Chandra
Amit
Chandra
Nigel M. Smith
Nigel M.
Smith
Lorraine E. Young
Lorraine E.
Young
David Williams
David
Williams
Chris Denning
Chris
Denning
Automated, scalable culture of human embryonic stem cells in feeder free conditions
Loughborough University
2009
Human embryonic stem cells
Automation
CompacT SelecT
Scalability
Process control
Mechanical Engineering not elsewhere classified
2009-02-17 16:49:52
Journal contribution
https://repository.lboro.ac.uk/articles/journal_contribution/Automated_scalable_culture_of_human_embryonic_stem_cells_in_feeder_free_conditions/9545834
Large-scale manufacture of human embryonic stem cells (hESCs) is prerequisite to their widespread use in biomedical applications. However, current hESC culture strategies are labor-intensive and employ highly variable processes, presenting challenges for scaled production and commercial development. Here we demonstrate that passaging of the hESC lines, HUES7, and NOTT1, with trypsin in feeder-free conditions, is compatible with complete automation on the CompacT SelecT, a commercially available and industrially relevant robotic platform. Pluripotency was successfully retained, as evidenced by consistent proliferation during serial passage, expression of stem cell markers (OCT4, NANOG, TRA1-81, and SSEA-4), stable karyotype, and multi-germlayer differentiation in vitro, including to pharmacologically responsive cardiomyocytes. Automation of hESC culture will expedite cell-use in clinical, scientific, and industrial applications.