Thomas, Rob Anderson, David Chandra, Amit Smith, Nigel M. Young, Lorraine E. Williams, David Denning, Chris Automated, scalable culture of human embryonic stem cells in feeder free conditions Large-scale manufacture of human embryonic stem cells (hESCs) is prerequisite to their widespread use in biomedical applications. However, current hESC culture strategies are labor-intensive and employ highly variable processes, presenting challenges for scaled production and commercial development. Here we demonstrate that passaging of the hESC lines, HUES7, and NOTT1, with trypsin in feeder-free conditions, is compatible with complete automation on the CompacT SelecT, a commercially available and industrially relevant robotic platform. Pluripotency was successfully retained, as evidenced by consistent proliferation during serial passage, expression of stem cell markers (OCT4, NANOG, TRA1-81, and SSEA-4), stable karyotype, and multi-germlayer differentiation in vitro, including to pharmacologically responsive cardiomyocytes. Automation of hESC culture will expedite cell-use in clinical, scientific, and industrial applications. Human embryonic stem cells;Automation;CompacT SelecT;Scalability;Process control;Mechanical Engineering not elsewhere classified 2009-02-17
    https://repository.lboro.ac.uk/articles/journal_contribution/Automated_scalable_culture_of_human_embryonic_stem_cells_in_feeder_free_conditions/9545834