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Catia Fidalgo
Catia
Fidalgo
Laura Iop
Laura
Iop
Manuela Sciro
Manuela
Sciro
M. Harder
M.
Harder
Dimosthenis Mavrilas
Dimosthenis
Mavrilas
Sotiris Korossis
Sotiris
Korossis
Andrea Bagno
Andrea
Bagno
Giorgio Palu
Giorgio
Palu
Paola Aguiari
Paola
Aguiari
Gino Gerosa
Gino
Gerosa
A sterilization method for decellularized xenogeneic cardiovascular scaffolds
Loughborough University
2019
Decellularization
Sterilization methodology
Pericardium
Xenogeneic scaffolds
Peracetic acid
Mechanical Engineering not elsewhere classified
2019-03-01 11:26:58
Journal contribution
https://repository.lboro.ac.uk/articles/journal_contribution/A_sterilization_method_for_decellularized_xenogeneic_cardiovascular_scaffolds/9547862
Decellularized xenogeneic scaffolds have shown promise to be employed as compatible and functional
cardiovascular biomaterials. However, one of the main barriers to their clinical exploitation is the lack
of appropriate sterilization procedures. This study investigated the efficiency of a two-step sterilization
method, antibiotics/antimycotic (AA) cocktail and peracetic acid (PAA), on porcine and bovine decellularized pericardium. In order to assess the efficiency of the method, a sterilization assessment protocol was
specifically designed, comprising: i) controlled contamination with a known amount of bacteria; ii) sterility test; iii) identification of contaminants through MALDI-TOF (Matrix-Assisted Laser Desorption/
Ionization Time-of-Flight) mass spectrometry and iv) quantification by the Most Probable Number
(MPN) method. This sterilization assessment protocol proved to be a successful tool to monitor and optimize the proposed sterilization method. The treatment with AA + PAA method provided sterile scaffolds
while preserving the structural integrity and biocompatibility of the decellularized porcine and bovine
tissues. However, surface properties and cellular adhesion resulted slightly impaired on porcine pericardium. This work developed a sterilization method suitable for decellularized pericardial scaffolds that
could be adopted for in vivo tissue engineering. Together with the proposed sterilization assessment protocol, this decontamination method will foster the clinical translation of decellularized xenogeneic substitutes.