2134/36734
Diana Maradze
Diana
Maradze
Andrew Capel
Andrew
Capel
Neil Martin
Neil
Martin
Mark Lewis
Mark
Lewis
Yufeng Zheng
Yufeng
Zheng
Yang Liu
Yang
Liu
In vitro investigation of cellular effects of magnesium and magnesium-calcium alloy corrosion products on skeletal muscle regeneration
Loughborough University
2019
Magnesium alloy
Corrosion
Biocompatibility
Muscle
Cellular response
In vitro
Mechanical Engineering not elsewhere classified
Mechanical Engineering
2019-02-01 10:05:45
Journal contribution
https://repository.lboro.ac.uk/articles/journal_contribution/In_vitro_investigation_of_cellular_effects_of_magnesium_and_magnesium-calcium_alloy_corrosion_products_on_skeletal_muscle_regeneration/9548696
Biodegradable magnesium (Mg) has garnered attention for its use in orthopaedic
implants due to mechanical properties that closely match to those of bone.
Studies have been undertaken to understand the corrosion behaviour of these
materials and their effects on bone forming cells. However, there is lack of
research on how the corrosion of these biomaterials affect surrounding tissues
such as skeletal muscle. Mg plays an important role in the structural and
functional properties of skeletal muscle. It is therefore important to investigate
the response of skeletal muscle cells to both soluble (Mg ions) and insoluble
(corrosion granules) corrosion products. Through in vitro studies it is possible to
observe the effects of corrosion products on myotube formation by the fusion of
single muscle precursor cells known as myoblasts. To achieve this goal, it is first
important to determine if these corrosion products are toxic to myotubes. Here it
was noted that although there was a slight decrement in cellular viability after
initial exposure, this soon recovered to control levels. A high Ca/Mg ratio
resulted in the formation of large myotubes and a low Ca/Mg ratio negatively
affected myotube maturation. Mg2+ and Ca2+ ions are important in the process of
myogenesis, the concentration of these ions and the ratio of the ions to each other
played a significant role in myotube cellular activity. The outcomes of this study could pave the way to a bio-informed and integrated approach to the design and
engineering of Mg-based orthopaedic implants.