Anharmonic acoustic effects during DNA hybridization on an electrochemical quartz crystal resonator Samantha Swarbrick Igor Efimov Victor P. Ostanin David Klenerman Alexandra Stolzing Sourav Ghosh 2134/32872 https://repository.lboro.ac.uk/articles/journal_contribution/Anharmonic_acoustic_effects_during_DNA_hybridization_on_an_electrochemical_quartz_crystal_resonator/9562988 The paper describes a sensor for single-stranded DNA (ssDNA) biomarker based on anharmonic acoustic signals arising during hybridization with complementary thiolated ssDNA functionalised on the gold electrode of an electrochemical quartz crystal resonator. The steps of sensor preparation and hybridization are carried out in an electrochemical microfluidic flowcell. While the electrochemical impedance spectroscopy does not allow a definitive interpretation, the changes in resonance frequency and third Fourier harmonic current of the resonator on actuation at the fundamental mode indicate formation of a flexibly bound layer. The functionalization and hybridization steps monitored by the anharmonic detection technique (ADT) are described with a simple model based on Duffing nonlinear equation. 2018-05-09 09:14:26 Electrochemical quartz crystal microbalance Acoustic detection of biomarkers Anharmonic oscillations Single-stranded DNA hybridization Electrochemical impedance Mechanical Engineering not elsewhere classified