Anharmonic acoustic effects during DNA hybridization on an electrochemical quartz crystal resonator
Samantha Swarbrick
Igor Efimov
Victor P. Ostanin
David Klenerman
Alexandra Stolzing
Sourav Ghosh
2134/32872
https://repository.lboro.ac.uk/articles/journal_contribution/Anharmonic_acoustic_effects_during_DNA_hybridization_on_an_electrochemical_quartz_crystal_resonator/9562988
The paper describes a sensor for single-stranded DNA (ssDNA) biomarker based on anharmonic acoustic signals arising during hybridization with complementary thiolated ssDNA functionalised on the gold electrode of an electrochemical quartz crystal resonator. The steps of sensor preparation and hybridization are carried out in an electrochemical microfluidic flowcell. While the electrochemical impedance spectroscopy does not allow a definitive interpretation, the changes in resonance frequency and third Fourier harmonic current of the resonator on actuation at the fundamental mode indicate formation of a flexibly bound layer. The functionalization and hybridization steps monitored by the anharmonic detection technique (ADT) are described with a simple model based on Duffing nonlinear equation.
2018-05-09 09:14:26
Electrochemical quartz crystal microbalance
Acoustic detection of biomarkers
Anharmonic oscillations
Single-stranded DNA hybridization
Electrochemical impedance
Mechanical Engineering not elsewhere classified