2134/13177
Elizabeth Ratcliffe
Elizabeth
Ratcliffe
Katie Glen
Katie
Glen
Victoria L. Workman
Victoria L.
Workman
Adrian J. Stacey
Adrian J.
Stacey
Rob Thomas
Rob
Thomas
A novel automated bioreactor for scalable process optimisation of haematopoietic stem cell culture
Loughborough University
2013
Haematopoietic stem cells
Suspension culture
Cell culture automation
Process development
Bioreactor
Mechanical Engineering not elsewhere classified
2013-09-19 13:22:15
Journal contribution
https://repository.lboro.ac.uk/articles/journal_contribution/A_novel_automated_bioreactor_for_scalable_process_optimisation_of_haematopoietic_stem_cell_culture/9563867
Proliferation and differentiation of haematopoietic stem cells (HSCs) from umbilical cord blood at large scale will potentially underpin production of a number of therapeutic cellular products in development, including erythrocytes and platelets. However, to achieve production processes that are scalable and optimised for cost and quality, scaled down development platforms that can define process parameter tolerances and consequent manufacturing controls are essential.
We have demonstrated the potential of a new, automated, 24 × 15 mL replicate suspension bioreactor system, with online monitoring and control, to develop an HSC proliferation and differentiation process for erythroid committed cells (CD71+, CD235a+). Cell proliferation was relatively robust to cell density and oxygen levels and reached up to 6 population doublings over 10 days. The maximum suspension culture density for a 48 h total media exchange protocol was established to be in the order of 107 cells/mL. This system will be valuable for the further HSC suspension culture cost reduction and optimisation necessary before the application of conventional stirred tank technology to scaled manufacture of HSC derived products.