2134/28346
Richard Ferguson
Richard
Ferguson
Julie E.A. Hunt
Julie E.A.
Hunt
Mark Lewis
Mark
Lewis
Neil Martin
Neil
Martin
Darren J. Player
Darren J.
Player
Carolin Stangier
Carolin
Stangier
Conor W. Taylor
Conor W.
Taylor
Mark Turner
Mark
Turner
The acute angiogenic signalling response to low-load resistance exercise with blood flow restriction
Loughborough University
2018
BFR
Capillaries
Hypoxia
Kaatsu
Resistance training
Mechanical Engineering
Medical and Health Sciences not elsewhere classified
2018-02-01 14:23:10
Journal contribution
https://repository.lboro.ac.uk/articles/journal_contribution/The_acute_angiogenic_signalling_response_to_low-load_resistance_exercise_with_blood_flow_restriction/9617153
This study investigated protein kinase activation and gene expression of angiogenic factors in response to low-load resistance exercise with or without blood flow restriction (BFR). In a repeated measures cross-over design, six males performed four sets of bilateral knee extension exercise at 20% 1RM (reps per set = 30:15:15:continued to fatigue) with BFR (110 mmHg) and without (CON). Muscle biopsies were obtained from the vastus lateralis before, 2 and 4 h post-exercise. mRNA expression was determined using real-time RT-PCR. Protein phosphorylation/expression was determined using Western blot. p38MAPK phosphorylation was greater (p = 0.05) at 2 h following BFR (1.3 ± 0.8) compared to CON (0.4 ± 0.3). AMPK phosphorylation remained unchanged. PGC-1α mRNA expression increased at 2 h (5.9 ± 1.3 vs. 2.1 ± 0.8; p = 0.03) and 4 h (3.2 ± 0.8 vs. 1.5 ± 0.4; p = 0.03) following BFR exercise with no change in CON. PGC-1α protein expression did not change following either exercise. BFR exercise enhanced mRNA expression of vascular endothelial growth factor (VEGF) at 2 h (5.2 ± 2.8 vs 1.7 ± 1.1; p = .02) and 4 h (6.8 ± 4.9 vs. 2.5 ± 2.7; p = .01) compared to CON. mRNA expression of VEGF-R2 and hypoxia-inducible factor 1α increased following BFR exercise but only eNOS were enhanced relative to CON. Matrix metalloproteinase-9 mRNA expression was not altered in response to either exercise. Acute low-load resistance exercise with BFR provides a targeted angiogenic response potentially mediated through enhanced ischaemic and shear stress stimuli.