No effect of 24 h severe energy restriction on appetite regulation and <i>ad libitum</i> energy intake in overweight and obese males David Clayton Mark Creese Nicola Skidmore David Stensel Lewis James 2134/22299 https://repository.lboro.ac.uk/articles/journal_contribution/No_effect_of_24_h_severe_energy_restriction_on_appetite_regulation_and_i_ad_libitum_i_energy_intake_in_overweight_and_obese_males/9629042 © 2016 Macmillan Publishers Limited, part of Springer Nature. Background/Objectives:Long-term success of weight loss diets might depend on how the appetite regulatory system responds to energy restriction (ER). This study determined the effect of 24 h severe ER on subjective and hormonal appetite regulation, subsequent ad libitum energy intake and metabolism.Subjects/Methods:In randomised order, eight overweight or obese males consumed a 24 h diet containing either 100% (12105 (1174 kJ; energy balance; EB) or 25% (3039 (295) kJ; ER) of estimated daily energy requirements (EER). An individualised standard breakfast containing 25% of EER (3216 (341) kJ) was consumed the following morning and resting energy expenditure, substrate utilisation and plasma concentrations of acylated ghrelin, glucagon-like peptide-1 (GLP-17–36), glucose-dependant insulinotropic peptide (GIP1–42), glucose, insulin and non-esterified fatty acid (NEFA) were determined for 4 h after breakfast. Ad libitum energy intake was assessed in the laboratory on day 2 and via food records on day 3. Subjective appetite was assessed throughout.Results:Energy intake was not different between trials for day 2 (EB: 14946 (1272) kJ; ER: 15251 (2114) kJ; P=0.623), day 3 (EB: 10580 (2457) kJ; 10812 (4357) kJ; P=0.832) or day 2 and 3 combined (P=0.693). Subjective appetite was increased during ER on day 1 (P<0.01), but was not different between trials on day 2 (P>0.381). Acylated ghrelin, GLP-17–36 and insulin were not different between trials (P>0.104). Post-breakfast area under the curve (AUC) for NEFA (P<0.05) and GIP1–42 (P<0.01) were greater during ER compared with EB. Fat oxidation was greater (P<0.01) and carbohydrate oxidation was lower (P<0.01) during ER, but energy expenditure was not different between trials (P=0.158).Conclusions:These results suggest that 24 h severe ER does not affect appetite regulation or energy intake in the subsequent 48 h. This style of dieting may be conducive to maintenance of a negative EB by limiting compensatory eating behaviour, and therefore may assist with weight loss.International Journal of Obesity advance online publication, 12 July 2016; doi:10.1038/ijo.2016.106. 2016-08-23 09:54:18 Appetite hormones Energy balance Calorie restriction Intermittent fasting Alternate day fasting Education Medical and Health Sciences not elsewhere classified