The effect of chronic high insulin exposure upon metabolic and myogenic markers in C2C12 skeletal muscle cells and myotubes.
Mark Turner
Darren J. Player
Neil Martin
Liz Akam
Mark Lewis
2134/28371
https://repository.lboro.ac.uk/articles/journal_contribution/The_effect_of_chronic_high_insulin_exposure_upon_metabolic_and_myogenic_markers_in_C2C12_skeletal_muscle_cells_and_myotubes_/9629984
Skeletal muscle is an insulin sensitive tissue and accounts for approximately 80% of
post-prandial glucose disposal. This study describes the effects of insulin, delivered
for 72 hours, to skeletal muscle myoblasts during differentiation or to skeletal muscle
myotubes. After chronic treatment, cultures were acutely stimulated with insulin and
analysed for total and phosphorylated Akt (Ser473), mRNA expression of metabolic
and myogenic markers and insulin-stimulated glucose uptake. Skeletal muscle cells
differentiated in the presence of insulin chronically, reduced acute insulin stimulated
phosphorylation of Akt Ser473. In addition, there was a reduction in mRNA expression
of Hexokinase II (HKII), GLUT4 and PGC-1α. Insulin-stimulated glucose uptake was
attenuated when cells were differentiated in the presence of insulin. In contrast,
myotubes exposed to chronic insulin showed no alterations in phosphorylation of Akt
Ser473. Both HKII and GLUT4 mRNA expression were reduced by chronic exposure
to insulin; while PGC-1α was not different between culture conditions and was
increased by acute insulin stimulation. These data suggest that there are differential
responses in insulin signalling, transcription and glucose uptake of skeletal muscle
cells when cultured in either the presence of insulin during differentiation or in myotube cultures.
2018-02-05 09:23:45
Hyperinsulineamia
Differentiation
Glucose uptake
Insulin signalling
Medical and Health Sciences not elsewhere classified