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A tunable nanopore sensor for the detection of metal ions using translocation velocity and biphasic pulses

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journal contribution
posted on 2016-11-08, 14:27 authored by Laura Mayne, Steven ChristieSteven Christie, Mark PlattMark Platt
A Tunable Resistive Pulse Sensor utilising a Polyurethane nanopore, has been used to characterise nanoparticles as they traverse the pore opening. Here we demonstrate that the translocation speed, conductive and resistive pulse magnitude, can be used to infer the surface charge of a nanoparticle, and act as a specific transduction signal for the binding of metal ions to ligands on the particles surface. Surfaces of silica nanoparticles were modified with a ligand to demonstrate the concept, and used to extract copper (II) ions (Cu2+) from solution. By tuning the pH and ionic strength of the solution, a biphasic pulse, a conductive followed by a resistive pulse is recorded. Biphasic pulses are becoming a powerful way to characterise materials, and provide an insight into the translocation mechanism, and here we present their first use to detect the presence of metal ions in solution. We demonstrate how combinations of translocation speed and/ or biphasic pulse behaviour are used to detect Cu2+ with quantitative responses across a range of pH and ionic strengths. Using a generic ligand this assay allows a clear signal for Cu2+ as low as 1 ppm with short 5 minute incubation time, and capable of measuring 10 ppm Cu2+ in the presence of 5 other ions. The method has potential for monitoring heavy metals in biological and environmental samples.

History

School

  • Science

Department

  • Chemistry

Published in

Nanoscale

Volume

8

Issue

45

Pages

19139 - 19147

Citation

MAYNE, L.J., CHRISTIE, S.D.R. and PLATT, M., 2016. A tunable nanopore sensor for the detection of metal ions using translocation velocity and biphasic pulses. Nanoscale, 8, 19139-19147.

Publisher

Royal Society of Chemistry

Version

  • AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Acceptance date

2016-10-27

Publication date

2016-10-27

Notes

This paper was accepted for publication in the journal Nanoscale and the definitive published version is available at http://dx.doi.org/10.1039/C6NR07224K

ISSN

2040-3372

Language

  • en

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