Butler_Revised CC Feature Article_final.pdf (1000.65 kB)
Application of lanthanide luminescence in probing enzyme activity
journal contribution
posted on 2018-10-01, 10:10 authored by Sarah Hewitt, Stephen ButlerStephen ButlerEnzymes play critical roles in the regulation of cellular function and are implicated in numerous disease conditions. Reliable and practicable assays are required to study enzyme activity, to facilitate the discovery of inhibitors and activators of enzymes related to disease. In recent years, a variety of enzyme assays have been devised that utilise luminescent lanthanide(iii) complexes, taking advantage of their high detection sensitivities, long luminescence lifetimes, and line-like emission spectra that permit ratiometric and time-resolved analyses. In this Feature article, we focus on recent progress in the development of enzyme activity assays based on lanthanide(iii) luminescence, covering a variety of strategies including Ln(iii)-labelled antibodies and proteins, Ln(iii) ion encapsulation within defined peptide sequences, reactivity-based Ln(iii) probes, and discrete Ln(iii) complexes. Emerging approaches for monitoring enzyme activity are discussed, including the use of anion responsive lanthanide(iii) complexes, capable of molecular recognition and luminescence signalling of polyphosphate anions.
Funding
This research was supported by the Wellcome Trust (204500/Z/16/Z).
History
School
- Science
Department
- Chemistry
Published in
Chem Commun (Camb)Citation
HEWITT, S.H. and BUTLER, S.J., 2018. Application of lanthanide luminescence in probing enzyme activity. Chemical Communications, 54, pp. 6635-6647.Publisher
© Royal Society of Chemistry (RSC)Version
- AM (Accepted Manuscript)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Acceptance date
2018-05-10Publication date
2018Notes
This paper was accepted for publication in the journal Chemical Communications and the definitive published version is available at https://doi.org/10.1039/c8cc02824a.ISSN
1359-7345eISSN
1364-548XPublisher version
Language
- en