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Bright mono-aqua europium complexes based on triazacyclononane that bind anions reversibly and permeate cells efficiently
journal contribution
posted on 2015-09-15, 15:38 authored by Stephen ButlerStephen Butler, Brian K. McMahon, Robert Pal, David Parker, James W. WaltonA series of five europium(III) complexes has been prepared from heptadentate N5O2 ligands that possess a brightness of more than 10 mM−1 cm−1 in water, following excitation over the range λ=330–355 nm. Binding of several oxy anions has been assessed by emission spectral titrimetric analysis, with the binding of simple carboxylates, lactate and citrate involving a common ligation mode following displacement of the coordinated water. Selectivity for bicarbonate allows the rapid determination of this anion in human serum, with Kd=37 mM (295 K). The complexes are internalised quickly into mammalian cells and exhibit a mitochondrial localisation at early time points, migrating after a few hours to reveal a predominant lysosomal distribution. Herein, we report the synthesis and complexation behaviour of strongly emissive europium (III) complexes that bind oxy-anions in aqueous media with an affinity and selectivity profile that is distinctively different from previously studied systems.
Funding
We thank the ERC (FCC 266804) and CISbio Bioassys (J.W.W.) for support.
History
School
- Science
Department
- Chemistry
Published in
CHEMISTRY-A EUROPEAN JOURNALVolume
19Issue
29Pages
9511 - 9517 (7)Citation
BUTLER, S.J. ... et al, 2013. Bright mono-aqua europium complexes based on triazacyclononane that bind anions reversibly and permeate cells efficiently. Chemistry - A European Journal, 19 (29), pp. 9511 - 9517.Publisher
© Wiley-VCH Verlag GmbH & Co.Version
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2013Notes
This article is closed access.ISSN
0947-6539Publisher version
Language
- en