Percival_s13104-020-4932-6.pdf (1.04 MB)
Determinations of the peroxidative susceptibilities of cod liver oils by a newly-developed 1H NMR-based method: resistance of an antioxidant-fortified product isolated from pre-fermented sources
journal contribution
posted on 2020-02-20, 11:53 authored by BC Percival, R Zbasnik, V Schlegel, Mark Edgar, J Zhang, M GrootveldObjective: To explore the molecular composition and antioxidant status of four natural (unrefined) cod liver oil (CLO)
products, three of which (Products 1–3) were non-fermented, whilst one (Product 4) was isolated from pre-fermented
cod livers, and hence was naturally antioxidant-fortified. Potential antioxidants and aldehyde-scavenging agents were
determined by recommended and/or 1H NMR methods; peroxyl radical-specific oxygen radical absorbance capacity
(ORAC) values were measured fluorimetrically. The activities of such antioxidants were also investigated by assessing
the susceptibilities/resistivities of these CLOs to thermo-oxidation by 1H NMR analysis, which monitored the timedependent evolution of aldehydic lipid oxidation products at 180 °C.
Results: Product 4 displayed much higher, albeit variable ORAC values (mean ± SEM 91.4 ± 19.5 mmol. trolox equivalents/kg) than those of Products 1–3, an observation arising from significant levels of peroxidation-blocking and/
or aldehyde-consuming collagenous polypeptides/peptides, flavonones, biogenic amines, total phenolics, tannins,
and ammoniacal agents therein. All of these agents were undetectable in Products 1–3. Quantitative considerations
indicated that collagenous gel agents (present at ca. 1.5% (w/w)) were the most powerful Product 4 antioxidants.
Significantly lower levels of aldehydes were generated in this product when exposed to thermal-stressing episodes.
Results confirmed the enhanced peroxidative resistivity of a pre-fermented, antioxidant-rich natural CLO over those of
corresponding non-fermented products. Product 4: Green Pasture Blue Ice™ fermented cod liver oil.
Funding
Weston A. Price Foundation (DC, USA
History
School
- Science
Department
- Chemistry
Published in
BMC Research NotesVolume
13Issue
1Publisher
Springer Science and Business Media LLCVersion
- VoR (Version of Record)
Rights holder
© The authorsPublisher statement
This is an Open Access Article. It is published by Springer under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/Acceptance date
2020-02-01Publication date
2020-02-12Copyright date
2020eISSN
1756-0500Publisher version
Language
- en
Depositor
Dr Mark Edgar Deposit date: 19 February 2020Article number
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