Wragg_biot.201900106.pdf (3.32 MB)
Development of a 3D tissue-engineered skeletal muscle and bone co‐culture system
journal contribution
posted on 2019-09-10, 15:24 authored by Nick Wragg, Diogo Mosqueira, Lia Blokpeol‐Ferreras, Andrew CapelAndrew Capel, Darren J Player, Neil MartinNeil Martin, Yang LiuYang Liu, Mark LewisMark LewisIn vitro three‐dimensional (3D) tissue engineered (TE) structures have been shown to better represent in vivo tissue morphology and biochemical pathways than monolayer culture, and are less ethically questionable than animal models. However, to create systems with even greater relevance, multiple integrated tissue systems should be recreated in vitro. In the present study, the effects and conditions most suitable for the co‐culture of TE skeletal muscle and bone were investigated. High‐glucose Dulbecco's Modified Eagle Medium (HG‐DMEM) supplemented with 20% foetal bovine serum (FBS) followed by HG‐DMEM with 2% horse serum was found to enable proliferation of both C2C12 muscle precursor cells and TE85 human osteosarcoma cells, fusion of C2C12s into myotubes, as well as an up‐regulation of RUNX2/CBFa1 in TE85s. Myotube formation was also evident within indirect contact monolayer cultures. Finally, in 3D co‐cultures, TE85 collagen/hydroxyapatite constructs had significantly greater expression of RUNX2/CBFa1 and osteocalcin/BGLAP in the presence of collagen‐based C2C12 skeletal muscle constructs; however, fusion within these constructs appeared reduced. This work demonstrates the first report of the simultaneous co‐culture and differentiation of 3D TE skeletal muscle and bone, and represents a significant step towards a full in vitro 3D musculoskeletal junction model.
Funding
Doctoral Training Centre of Regenerative Medicine, (EPSRC, UK)
History
School
- Mechanical, Electrical and Manufacturing Engineering
- Sport, Exercise and Health Sciences
Published in
Biotechnology JournalVolume
15Issue
1Pages
1900106Publisher
WileyVersion
- VoR (Version of Record)
Rights holder
© The AuthorsPublisher statement
This is an Open Access Article. It is published by Wiley under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/Acceptance date
2019-07-19Publication date
2019-09-20Copyright date
2019ISSN
1860-6768eISSN
1860-7314Publisher version
Language
- en
Depositor
Dr Nick WraggArticle number
1900106Usage metrics
Categories
No categories selectedLicence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC