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Development of a mini 3D cell culture system using well defined nickel grids for the investigation of cell scaffold interactions

journal contribution
posted on 2017-05-12, 11:21 authored by Tao SunTao Sun, Rod Smallwood, Sheila MacNeil
A bioreactor system was developed using a series of fine mesh nickel grids as free standing scaffolds to investigate the behaviours of fibroblasts and keratinocytes in tissue culture. It was found that the mesh size of the suspended grids, but not of the grids that attached to tissue culture surface, had significant influences on cell behaviour and there was a maximum size for fibroblast to span within the defined culture period. Time lapse video microscopy demonstrated fibroblasts cultured on these grids initially migrated onto the struts but then worked together to fill in the voids between struts with a membranous sheet of tissue. In contrast keratinocytes barely migrated from the initial site of cell deposition and when they moved (to a modest extent) it was as an integrated sheet of cells. Similar results were observed when both types of cells were co-cultured in the system.

Funding

This work was financially supported by the EPSRC.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Journal of Materials Science: Materials in Medicine

Volume

20

Issue

7

Pages

1483 - 1493

Citation

SUN, T., SMALLWOOD, R. and MACNEIL, S., 2009. Development of a mini 3D cell culture system using well defined nickel grids for the investigation of cell scaffold interactions. Journal of Materials Science: Materials in Medicine, 20 (7), pp.1483-1493

Publisher

© Springer Science+Business Media, LLC

Version

  • NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2009

Notes

This paper is closed access.

ISSN

0957-4530

eISSN

1573-4838

Language

  • en

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