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effect-of-mechanical-loading-on-osteogenesis-of-human-embryonic-stem-cellderived-mesenchymal-progenitors-within-collagen-microsphe-2157-7013-1000282.pdf (1.66 MB)

Effect of mechanical loading on osteogenesis of human embryonic stem cell-derived mesenchymal progenitors within collagen microspheres

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journal contribution
posted on 2018-10-16, 10:38 authored by Maryam Shariatzadeh, A. Baldit, C.M. Perrault, D. Lacroix
Mechanical forces and 3D topological environment can be used to control differentiation of mesenchymal stem cells. However, mesenchymal stem cell fate determined by the effect of physical and mechanical cues is not yet fully understood. Understanding how mechanical cues in the microenvironment orchestrate stem cell differentiation provides valuable insight that can be used to improve current techniques in cell therapy. This study investigates the osteogenic effect of mechanical stimulations on soft cellular microspheres loaded with human embryonic stem cellderived mesenchymal progenitors (hES-MPs) when subjected to dynamic loading and in the absence of chemical stimulation. Microspheres were produced by gelation of bovine collagen type I with 1000 to 2000 hES-MP cells seeded per droplet. Four loading conditions were studied: (1) 10% constant strain was applied by a Bose biodynamic bioreactor for 15 min/day or 40 min/day for 5 or 10 days respectively; (2) 10% adjusted strain was applied (subtraction of polydimethylsiloxane (PDMS) plastic elongation from global strain) using Bose biodynamic bioreactor for the same 4 duration/conditions as in the constant strain protocol. The results indicate that applying mechanical stimulation to hES-MPs/collagen microspheres induced osteogenic differentiation of cells when the loading protocol was adjusted. Alkaline phosphatase activity of samples in the adjusted loading protocol increased significantly on day 14 whilst, the deposited minerals, matrix reorganisation and alignment of collagen fibres enhanced from day 21 post encapsulation onward. Application of cyclic loading to 3D culture of hES-MP cells can be used as a model to regulate mechanostimulation and linage differentiation in vitro.

Funding

Financial support from the European Research Council (258321) is acknowledged.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Journal of Cell Science & Therapy

Volume

9

Issue

3

Citation

SHARIATZADEH, M. ... et al, 2018. Effect of mechanical loading on osteogenesis of human embryonic stem cell-derived mesenchymal progenitors within collagen microspheres. Journal of Cell Science & Therapy, 9 (3), 1000282.

Publisher

OMICS International © The Authors

Version

  • VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/ by/4.0/

Publication date

2018

Notes

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

ISSN

2157-7013

Language

  • en