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Effects of combined cryopreservation and decellularization on the biomechanical, structural and biochemical properties of porcine pulmonary heart valves
journal contribution
posted on 2019-03-01, 13:45 authored by Karolina Theodoridis, Janina Muller, Robert Ramm, Katja Findeisen, Birgit Andree, Sotiris KorossisSotiris Korossis, Axel Haverich, Andres HilfikerNon-fixed, decellularized allogeneic heart valve scaffolds seem to be the best choice for heart valve
replacement, their availability, however, is quite limited. Cryopreservation could prolong their
shelf-life, allowing for their ideal match to a recipient. In this study, porcine pulmonary valves were
decellularized using detergents, either prior or after cryopreservation, and analyzed. Mechanical integrity
was analyzed by uniaxial tensile testing, histoarchitecture by histological staining, and composition by
DNA, collagen (hydroxyproline) and GAG (chondroitin sulfate) quantification. Residual sodium dodecyl
sulfate (SDS) in the scaffold was quantified by applying a methylene blue activation assay (MBAS).
Cryopreserved decellularized scaffolds (DC) and scaffolds that were decellularized after cryopreservation
(CD) were compared to fresh valves (F), cryopreserved native valves (C), and decellularized only scaffolds
(D). The E-modulus and tensile strength of decellularized (D) tissue showed no significant difference
compared to DC and CD. The decellularization resulted in an overall reduction of DNA and GAG, with
DC containing the lowest amount of GAGs. The DNA content in the valvular wall of the CD group was
higher than in the D and DC groups. CD valves showed slightly more residual SDS than DC valves, which
might be harmful to recipient cells. In conclusion, cryopreservation after decellularization was shown to
be preferable over cryopreservation before decellularization. However, in vivo testing would be necessary
to determine whether these differences are significant in biocompatibility or immunogenicity of the
scaffolds
Funding
This work was funded by the German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) via the Cluster of Excellence ‘From regenerative biology to reconstructive therapy’ (REBIRTH) and via the C7 project of TRR127.
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
Acta BiomaterialiaVolume
43Pages
71 - 77Citation
THEODORIDIS, K. ... et al., 2016. Effects of combined cryopreservation and decellularization on the biomechanical, structural and biochemical properties of porcine pulmonary heart valves. Acta Biomaterialia, 43, pp. 71 - 77.Publisher
© Acta Materialia Inc. Published by Elsevier Ltd.Version
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2016-07-12Notes
This paper is in closed access.ISSN
1742-7061Publisher version
Language
- en