Thesis-1991-French.pdf (7.04 MB)
Fluorescence immunoassay for cyclosporin A
thesis
posted on 2018-06-01, 15:03 authored by Martin T. FrenchMonodansylcadavarine (MDC) was used to synthesise a fluorescent derivative of cyclosporin A and
the product of the reaction was isolated by preparative thin layer chromatography (TLC) and
purified by high performance liquid chromatography (HPLC). The fluorescent derivative was shown
to bind with a polyclonal antibody to cyclosporin A by submitting the derivative for analysis by
cyclosporin radioimmunoassay (RIA). However this derivative did not bind with a monoclonal
antibody used in a RIA specific for the parent compound. To achieve this fluorescent derivatives
were synthesised using cyclosporin-C-hemisuccinate as the staring material with MDC, 4-bromomethyl-7-methoxycoumarin (BMMC), 4-bromomethyl-6,7-dimethoxycoumarin (BMDC) and
tetramethyl rhodaminecadavarine (TRC) as the labels. All derivatives were isolated and purified by
TLC and HPLC and shown to have antibody binding in the parent compound specific RIA. The
fluorescent properties of the derivatives were investigated and the most promising, BMMC and TRC
used in the immunoassay development. [Continues.]
History
School
- Science
Department
- Chemistry
Publisher
© Martin FrenchPublisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
1991Notes
A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy at Loughborough University.Language
- en