Cosma_elife-50936-v2.pdf (2.17 MB)
Identifying prostate cancer and its clinical risk in asymptomatic men using machine learning of high dimensional peripheral blood flow cytometric natural killer cell subset phenotyping data
journal contribution
posted on 2020-06-25, 10:26 authored by Simon Hood, Georgina CosmaGeorgina Cosma, Gemma Foulds A., Catherine Johnson, Stephen Reeder, Stéphanie McArdle E., Masood Khan, Graham Pockley A.We demonstrate that prostate cancer can be identified by flow cytometric profiling of blood immune cell subsets. Herein, we profiled natural killer (NK) cell subsets in the blood of 72 asymptomatic men with Prostate Specific Antigen (PSA) levels < 20ng ml, of whom 31 had benign disease (no cancer) and 41 had prostate cancer. Statistical and computational methods identified a panel of eight phenotypic features(CD56 dimCD16high,
CD56+DNAM-1-, CD56+LAIR-1+, CD56+LAIR-1-,
CD56BRIGHTCD8+, CD56+NKp30+, CD56+NKp30-,
CD56+NKp46+) which, when incorporated into an Ensemble machine learning prediction model, distinguished between the presence of benign prostate disease and prostate cancer. The machine learning model was then adapted to predict the D’Amico Risk Classification using data from 54 patients with prostate cancer and was shown to accurately differentiate between the presence of low/intermediate risk disease and high risk disease without the need for additional clinical data. This simple blood test has the potential to transform prostate cancer diagnostics
Funding
John and Lucille van Geest Foundation
The Leverhulme Trust (Research Project Grant RPG-2016-252)
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eLifeVolume
9Pages
e50936Publisher
eLife Sciences Publications LtdVersion
- VoR (Version of Record)
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© The authorsPublisher statement
This is an Open Access Article. It is published by Elife under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/Acceptance date
2020-06-25Publication date
2020-07-28Copyright date
2020ISSN
2050-084XPublisher version
Depositor
Dr Georgina Cosma. Deposit date: 25 June 2020Article number
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