Manuscript revised version for Lboro repository.pdf (883.99 kB)
Impact of aerobic exercise and fatty acid supplementation on global and gene-specific DNA methylation
journal contribution
posted on 2019-04-24, 10:31 authored by David J. Hunter, Lynsey James, Bethan Hussey, Alex Wadley, Martin Lindley, Sarabjit MastanaSarabjit MastanaLifestyle interventions, including exercise and dietary supplementation, can modify DNA methylation and exert health benefits; however, the underlying mechanisms are poorly understood. Here we investigated the impact of acute aerobic exercise and the supplementation of omega-3 polyunsaturated fatty acids (n-3 PUFA) and extra virgin olive oil (EVOO) on global and gene-specific (PPARGC1A, IL6 and TNF) DNA methylation, and DNMT mRNA expression in leukocytes of disease-free individuals. Eight trained male cyclists completed an exercise test before and after a four-week supplementation of n-3 PUFA and EVOO in a double-blind, randomised, repeated measures design. Exercise triggered global hypomethylation (Pre 79.2%; Post 78.7%; p = 0.008), alongside, hypomethylation (Pre 6.9%; Post 6.3%; p < 0.001) and increased mRNA expression of PPARGC1A (p < 0.001). Associations between PPARGC1A methylation and exercise performance were also detected. An interaction between supplement and trial was detected for a single CpG of IL6 indicating increased DNA methylation following n-3 PUFA and decreased methylation following EVOO (p = 0.038). Global and gene-specific DNA methylation associated with markers of inflammation and oxidative stress. The supplementation of EVOO reduced DNMT1 mRNA expression compared to n-3 PUFA supplementation (p = 0.048), whereas, DNMT3a (p=0.018) and DNMT3b (p=0.046) mRNA expression were decreased following exercise. In conclusion, we demonstrate that acute exercise and dietary supplementation of n-3 PUFAs and EVOO induce DNA methylation changes in leukocytes, potentially via the modulation of DNMT mRNA expression. Future studies are required to further elucidate the impact of lifestyle interventions on DNA methylation.
History
School
- Sport, Exercise and Health Sciences
Published in
EpigeneticsVolume
14Issue
3Pages
294-309Citation
HUNTER, D.J. ... et al, 2019. Impact of aerobic exercise and fatty acid supplementation on global and gene-specific DNA methylation. Epigenetics, 14 (3), pp.294-309.Publisher
© Taylor and FrancisVersion
- AM (Accepted Manuscript)
Publisher statement
This is an Accepted Manuscript of an article published by Taylor & Francis in Epigenetics on 18 March 2019, available online: http://www.tandfonline.com/10.1080/15592294.2019.1582276.Acceptance date
2019-02-07Publication date
2019-03-18ISSN
1559-2294eISSN
1559-2308Publisher version
Language
- en