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In situ image analysis of interactions between normal human keratinocytes and fibroblasts cultured in three-dimensional fibrin gels
journal contribution
posted on 2017-05-12, 12:52 authored by Tao SunTao Sun, John W. Haycock, Sheila MacNeilThe non-invasive investigation of different cells to interact and become spatially organised in a three-dimensional (3D) environment or
scaffold is an important challenge in tissue engineering and tissue physiology. The aim of the present study was to develop 3D cell culture
systems using fibrin gels, which would allow for the single and co-culture of different cell types with in situ image analysis. Two chambers
were constructed for mono-culture and co-culture of human dermal fibroblasts and keratinocytes. During cell culture, in situ imaging
and morphological characterisation of cells was assessed using brightfield light and/or fluorescence microscopy, and later confirmed by
staining of fixed cells using immunofluorescence microscopy. The results showed that it was possible to investigate fibroblast and
keratinocyte interactions in a fibrin scaffold for at least 12 days. Using this model system it was found that when a co-culture of
fibroblasts and keratinocytes were plated on top of the fibrin gels, fibroblasts were seen to migrate into the gels within 2–3 days in
contrast to keratinocytes, which did not enter. However, keratinocytes were found to retard fibroblast migration into gels when
compared to fibroblasts cultured on their own, illustrating the dependency of intracellular communication on cell position for
reconstructive approaches.
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
BiomaterialsVolume
27Issue
18Pages
3459 - 3465Citation
SUN, T., HAYCOCK, J.W. and MACNEIL, S., 2006. In situ image analysis of interactions between normal human keratinocytes and fibroblasts cultured in three-dimensional fibrin gels. Biomaterials, 27 (18), pp.3459-3465Publisher
© Elsevier Ltd.Version
- NA (Not Applicable or Unknown)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2006Notes
This paper is closed access.ISSN
0142-9612Publisher version
Language
- en