ac-2016-043156 Arthur et al_accepted manuscript and SI.pdf (925.89 kB)
0/0

Increasing peak capacity in nontargeted omics applications by combining full scan field asymmetric waveform ion mobility spectrometry with liquid chromatography–mass spectrometry

Download (925.89 kB)
journal contribution
posted on 26.04.2017 by Kayleigh L. Arthur, Matthew Turner, Jim Reynolds, Colin Creaser
Full scan field asymmetric waveform ion mobility spectrometry (FAIMS) combined with liquid chromatography and mass spectrometry (LC-FAIMS-MS) is shown to enhance peak capacity for omics applications. A miniaturized FAIMS device capable of rapid compensation field scanning has been incorporated into an ultrahigh performance liquid chromatography (UHPLC) and time-of-flight mass spectrometry analysis, allowing the acquisition of full scan FAIMS and MS nested data sets within the time scale of a UHPLC peak. Proof of principle for the potential of scanning LC-FAIMS-MS in omics applications is demonstrated for the nontargeted profiling of human urine using a HILIC column. The high level of orthogonality between FAIMS and MS provides additional unique compound identifiers with detection of features based on retention time, FAIMS dispersion field and compensation field (DF and CF), and mass-to-charge (m/z). Extracted FAIMS full scan data can be matched to standards to aid the identification of unknown analytes. The peak capacity for features detected in human urine using LC-FAIMS-MS was increased approximately threefold compared to LC-MS alone due to a combination of the reduction of chemical noise and separation of coeluting isobaric species across the entire analytical space. The use of FAIMS-selected in source collision induced dissociation (FISCID) yields fragmentation of ions, which reduces sample complexity associated with overlapping fragmentation patterns and provides structural information on the selected precursor ions.

Funding

The authors thank Owlstone Limited and Loughborough University for financial support.

History

School

  • Science

Department

  • Chemistry

Published in

Analytical Chemistry

Volume

89

Issue

6

Pages

3452 - 3459

Citation

ARTHUR, K.L. ... et al, 2017. Increasing peak capacity in nontargeted omics applications by combining full scan field asymmetric waveform ion mobility spectrometry with liquid chromatography–mass spectrometry. Analytical Chemistry, 89 (6), pp. 3452-3459.

Publisher

© American Chemical Society

Version

AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Acceptance date

23/02/2017

Publication date

2017-02-23

Copyright date

2017

Notes

This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.analchem.6b04315.

ISSN

0003-2700

eISSN

1520-6882

Language

en

Exports