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Influence of topography of nanofibrous scaffolds on functionality of engineered neural tissue
journal contribution
posted on 2018-05-30, 10:46 authored by Hadi Hajiali, Andrea Contestabile, Elisa MeleElisa Mele, Athanassia AthanassiouProperly engineered scaffolds combined with functional neurons can be instrumental for the effective repair of the neural tissue. In particular, it is essential to investigate how three-dimensional (3D) systems and topographical features can impact on neuronal activity to obtain engineered functional neural tissues. In this study, polyphenylene sulfone (PPSu) scaffolds constituted by randomly distributed or aligned electrospun nanofibers were fabricated to evaluate the neural activity in 3D culture environments for the first time. The obtained results demonstrated that the nanofibers can successfully support the adhesion and growth of neural stem cells (NSCs) and enhance neuronal differentiation compared to 2D substrates. In addition, NSCs could spread and migrate along the aligned fibers. The percentage of active NSC-derived neurons and the overall network activity in the fibrous substrates were also remarkably enhanced. Finally, the data of neuronal activity showed not only that the neurons cultured on the nanofibers are part of a functional network, but also that their activity increases, and the direction of neural signals can be controlled in the aligned 3D scaffolds.
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Materials
Published in
Journal of Materials Chemistry BVolume
6Issue
6Pages
930 - 939Citation
HAJIALI, H. ... et al, 2018. Influence of topography of nanofibrous scaffolds on functionality of engineered neural tissue. Journal of Materials Chemistry B, 6 (6), pp.930-939.Publisher
© The Royal Society of ChemistryVersion
- AM (Accepted Manuscript)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2018Notes
This paper was published in the journal Journal of Materials Chemistry and the definitive published version is available at https://doi.org/10.1039/c7tb02969a.ISSN
2050-750XeISSN
2050-7518Publisher version
Language
- en