Muzard et al Small March 2012 GUL final (2).pdf (1.89 MB)
M13 bacteriophage-activated superparamagnetic beads for affinity separation
journal contribution
posted on 2013-01-02, 14:05 authored by Julien Muzard, Mark PlattMark Platt, Gil U. LeeThe growth of the biopharmaceutical industry has created a demand for new technologies for
the purification of genetically engineered proteins. The efficiency of large-scale, highgradient
magnetic fishing could be improved if magnetic particles offering higher binding
capacity and magnetization were available. This article describes several strategies for
synthesizing SPMs that are composed of a M13 bacteriophage layer assembled on a
superparamagnetic core. Chemically cross-linking the pVIII proteins to a carboxyl
functionalized SPM produced highly responsive superparamagnetic particles with a side-on
oriented, adherent virus monolayer. Also, the genetic manipulation of the pIII proteins with a
His6 peptide sequence allowed reversible assembly of the bacteriophage on a nitrilotriacetic
acid functionalized core in an end-on configuration. These phage-magnetic particles were
successfully used to separate antibodies from high-protein concentration solutions in a single
step with a > 90 % purity. The dense magnetic core of these particles makes them five times
more responsive to magnetic fields than commercial materials composed of polymer-iron
oxide composites and a monolayer of phage could produced a 1000 fold higher antibody
binding capacity. These new bionanomaterials appear to be well-suited to large-scale highgradient
magnetic fishing separation and promise to be cost effective as a result of the selfassembling
and self-replicating properties of genetically engineered M13 bacteriophage.
History
School
- Science
Department
- Chemistry
Citation
MUZARD, J., PLATT, M. and LEE, G.U., 2012. M13 bacteriophage-activated superparamagnetic beads for affinity separation. Small, 8 (15), pp.2403-2411.Publisher
© WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimVersion
- SMUR (Submitted Manuscript Under Review)
Publication date
2012Notes
This article was submitted for publication in the journal, Small. It is the pre-peer reviewed version of the article.ISSN
1613-6810eISSN
1613-6829Publisher version
Language
- en