Thesis-1985-Wilmott.pdf (2.65 MB)
Metals as labels in immunoassay
thesis
posted on 2017-05-25, 14:12 authored by N.J. WilmottThe basic principles of immunoassay were first
reported by Berson and Yalow (1959) and since then it
has become an extremely powerful technique in the
determination of a broad spectrum of compounds. The
power of the technique lying chiefly in the areas of
specificity and versatility.
The principles of immunoassay are basically
straightforward. If the substance of analytical
interest is foreign to an animal, typically a rabbit,
sheep or goat, injection of that substance into the
animal will cause the production of a glycoprotein,
known as an antibody (Ab). The antibody produced will
have a specificity for the substance that initiated its
production, the antigen (Ag). Antigens are generally
naturally occur~ng macromolecules, e.g., proteins,
polysaccharides, nucleic acids, etc. Smaller molecules,
e.g., drugs, hormones, peptides, etc., do not
themselves initiate antibody production, but when
coupled to a macromolecular carrier, e.g., a protein or
a synthetic polypeptide, antibody production may be
initiated. The resultant antibodies will react with the
carrier linked molecule and also the small molecule
alone. A small molecule of this type is known as a
hapten.
History
School
- Science
Department
- Chemistry
Publisher
© N.J. WilmottPublisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
1985Notes
A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy of Loughborough University.Language
- en