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Microdroplet-based multiplex PCR on chip to detect foodborne bacteria producing biogenic amines
journal contribution
posted on 2015-06-11, 12:03 authored by Anna G. Sciancalepore, Elisa MeleElisa Mele, Valentina Arcadio, Francesco Reddavide, Francesco Grieco, Giuseppe Spano, Patrick Lucas, Giovanni Mita, Dario PisignanoThe development of fast, reliable and culture-independent molecular tools to detect bacteria producing
biogenic amines deserves the attention of research and ultimately of the food industry in order to protect
consumers’ health. Here we present the application of a simple, low-cost, fast and sensitive method to
perform microdroplet-based multiplex PCR, directly on a food matrix, for the simultaneous detection of
bacterial genes involved in biogenic amine biosynthesis. After inoculating wine with Lactobacillus brevis
IOEB 9809, cell lysis and DNA amplification are performed in one single step, without preliminary nucleic
acid extraction or purification treatments. The assay is performed in about 30 min, requiring 150 nL of
starting sample and it enables the detection of down to 15 bacterial cells. With respect to traditional
culture techniques, the speed, the simplicity and the cheapness of this procedure allow an effective
monitoring of microbial cells during food-making and processing.
Funding
G.S. and P.L. were supported by the European Union project BIAMFOOD/211441 (Controlling Biogenic Amines in Traditional Food Fermentations in Regional Europe).
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Materials
Published in
FOOD MICROBIOLOGYVolume
35Issue
1Pages
10 - 14 (5)Citation
SCIANCALEPORE, A.G. ... et al, 2013. Microdroplet-based multiplex PCR on chip to detect foodborne bacteria producing biogenic amines. Food Microbiology, 35 (1), pp. 10 - 14.Publisher
© Elsevier LtdVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2013Notes
This article is closed access.ISSN
0740-0020Publisher version
Language
- en