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Modeling the effect of exogenous calcium on keratinocyte and HaCat Cell proliferation and differentiation using an agent-based computational paradigm
journal contribution
posted on 2017-05-12, 13:03 authored by Dawn Walker, Tao SunTao Sun, Sheila MacNeil, Rod SmallwoodIn this study we sought to develop a computational modeling paradigm in order to describe the influence
of calcium on normal and transformed keratinocyte proliferation and differentiation. Keratinocytes and
HaCat cells were grown in monolayer cultures with low and physiologic calcium concentrations, and
levels of proliferation and involucrin expression were assessed. Both types of cells grew as monolayers
under a low-calcium environment, and stratified in media with physiologic levels of calcium. However,
keratinocytes were more proliferative in low rather than physiologic levels of calcium, whereas the
opposite was true for HaCat cells. Normal keratinocytes differentiated as calcium levels increased. HaCat
cells showed little differentiation at any calcium concentration. However, while the computer simulation
could be modified to describe the effect of calcium on the growth of normal keratinocytes, our findings
did not support the hypothesis that simply ‘‘turning off’’ the ability of HaCat cells to differentiate would
account for the growth characteristics of these transformed cells. This demonstrates the application of
computational modeling to hypothesis testing in biological systems
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Tissue EngineeringVolume
0Issue
0Pages
060913044658037 - 060913044658037Citation
WALKER, D. ... et al, 2006. Modeling the effect of exogenous calcium on keratinocyte and HaCat cell proliferation and differentiation using an agent-based computational paradigm. Tissue Engineering, 12 (8), pp.2301-2309Publisher
© Mary Ann Liebert, Inc. publishers.Version
- NA (Not Applicable or Unknown)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2006Notes
This paper is closed access.ISSN
1076-3279Publisher version
Language
- en