Algal Bisabolene_Clean.pdf (795.81 kB)
Photocatalytic production of bisabolene from green microalgae mutant: process analysis and kinetic modeling
journal contribution
posted on 2018-12-05, 15:58 authored by Irina Harun, Ehecatl Antonio del Rio-Chanona, Jonathan WagnerJonathan Wagner, Kyle J. Lauersen, Dongda Zhang, Klaus HellgardtCurrently, algal fuel research has commenced to shift toward genetically engineered mutants able to express and excrete desired products directly into the culture. In this study, a mutant strain of Chlamydomonas reinhardtii, engineered for bisabolene (alternative biodiesel) excretion, was cultivated at different illumination and temperatures to investigate their effects on cell growth and bisabolene production. Moreover, a kinetic model was constructed to identify the desirable conditions for biofuel synthesis. Three original contributions were concluded. First, this work confirmed that bisabolene was partially synthesized independently of biomass growth, indicating its feasibility for continuous production. Second, it was found that while bisabolene synthesis was independent of light intensity, it was strongly affected by temperature, resulting in conflicting desirable conditions for cell growth and product synthesis. Finally, through model prediction, optimal operating conditions were identified for mutant growth and bisabolene synthesis. This study therefore paves the way toward chemostat production and process scale-up.
Funding
This project received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement 640720. This project also received funding from the EPSRC project (EP/P016650/1, P65332). I.H. acknowledges funding from the Ministry of Higher Education, Malaysia and University Putra Malaysia.
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Industrial & Engineering Chemistry ResearchCitation
HARUN, I. ... et al, 2018. Photocatalytic production of bisabolene from green microalgae mutant: process analysis and kinetic modeling. Industrial & Engineering Chemistry Research, 57 (31), pp.10336–10344.Publisher
© American Chemical SocietyVersion
- AM (Accepted Manuscript)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Acceptance date
2018-07-03Publication date
2018-07-05Notes
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Industrial & Engineering Chemistry Research, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/10.1021/acs.iecr.8b02509.ISSN
0888-5885Publisher version
Language
- en