Preparation of liposomes. A novel application of microengineered membranes.pdf (1.03 MB)

Preparation of liposomes: a novel application of microengineered membranes

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journal contribution
posted on 18.04.2013, 15:21 by Abdallah Laouini, Catherine Charcosset, Hatem Fessi, Richard Holdich, Goran Vladisavljevic
Liposomes with a mean size of 59–308 nm suitable for pulmonary drug delivery were prepared by the ethanol injection method using nickel microengineered flat disc membranes with a uniform pore size of 5–40 μm and a pore spacing of 80 or 200 μm. An ethanolic phase containing 20–50 mg ml−1 phospholipid (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) or Lipoid® E80), 5–12.5 mg ml−1 stabilizer (cholesterol, stearic acid or cocoa butter), and 0 or 5 mg ml−1 vitamin E was injected through the membrane into an agitated aqueous phase at a controlled flux of 142–355 l m−2 h−1 and a shear stress on the membrane surface of 0.80–16 Pa. The mean particle size obtained under optimal conditions was 84 and 59 nm for Lipoid E80 and POPC liposomes, respectively. The particle size of the prepared liposomes increased with an increase in the pore size of the membrane and decreased with an increase in the pore spacing. Lipoid E80 liposomes stabilized by cholesterol or stearic acid maintained their initial size within 3 months. A high entrapment efficiency of 99.87% was achieved when Lipoid E80 liposomes were loaded with vitamin E. Transmission electron microscopy images revealed spherical multi-lamellar structure of vesicles. The reproducibility of the developed fabrication method was high.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Citation

LAOUINI, A. ... et al., 2013. Preparation of liposomes: a novel application of microengineered membranes. RSC Advances, 3 (15), pp. 4985 - 4994.

Publisher

© Royal Society of Chemistry

Version

AM (Accepted Manuscript)

Publication date

2013

Notes

This article was accepted for publication in the journal, RSC Advances [© Royal Society of Chemistry] and the definitive version is available at: http://dx.doi.org/10.1039/C3RA23411H

ISSN

2046-2069

Language

en

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