Rapid Analysis of N-methylpyrrolidine- IJIMS_FINAL2 and NMP-IMS supplementary information .pdf (318.08 kB)
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Rapid analysis of N-methylpyrrolidine in cefepime with thermal desorption ion mobility spectrometry

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journal contribution
posted on 09.03.2017 by Jim Reynolds, Laura Giddings, Ime C. Usen, Haodong Cheng, Junmin Shan, Matthew Turner, Colin Creaser
N-methyl-pyrrolidine (NMP) a potential impurity in the cephalosporin antibiotic cefepime is analysed using a rapid thermal desorption – ion mobility spectrometry (TD-IMS) method. The thermal desorption approach is shown to be capable of rapidly extracting NMP from the cefepime at 80 °C without causing thermal degradation of the cefepime. The ion mobility method has an analysis time of 1 min and demonstrates good linearity over a range from 0.3–3.0 μg ml−1 of NMP, with limits of detection and quantification of 0.056 and 0.1875 μg ml−1 respectively. The developed method was applied to the analysis of a cefepime sample and determined that NMP was present in a cefepime sample at a level of 0.0376 % with a percentage relative standard deviation (n = 6) of 3.2 %. This was compared with a LC-UV method which was in close agreement measuring NMP at 0.0384 % in the cefepime sample with a percentage RSD (n = 6) of 5.7 %. These results show that the TD-IMS method gives comparable data to the established LC methods and demonstrates the potential of TD-IMS for rapid measurement of volatile compounds in pharmaceutical matrices.

History

School

  • Science

Department

  • Chemistry

Published in

International Journal for Ion Mobility Spectrometry

Volume

19

Issue

4

Pages

209 - 217

Citation

REYNOLDS, J.C. ... et al, 2016. Rapid analysis of N-methylpyrrolidine in cefepime with thermal desorption ion mobility spectrometry. International Journal for Ion Mobility Spectrometry, 19 (4), pp. 209-217.

Publisher

© Springer

Version

AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Acceptance date

29/10/2016

Publication date

2016-11-08

Notes

The final publication is available at link.springer.com via http://dx.doi.org/10.1007/s12127-016-0210-7

ISSN

1435-6163

eISSN

1865-4584

Language

en

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