Smith et al_NMP in cefepime_Analytical Methods 2015_accepted manuscript.pdf (515.57 kB)
Rapid determination of N-Methylpyrrolidine in Cefepime by combining direct infusion electrospray ionisation-time-of-flight mass spectrometry with field asymmetric waveform ion mobility spectrometry
journal contribution
posted on 2016-03-15, 12:35 authored by Robert W. Smith, Lisa B. Cox, Aswandi Yudin, Jim ReynoldsJim Reynolds, Mark Powell, Colin CreaserThe determination of N-methyl pyrrolidine, a potential impurity in the cephalosporin antibiotic cefepime, by direct infusion ESI combined with field asymmetric waveform ion mobility spectrometry-mass spectrometry (ESI-FAIMS-MS) is demonstrated. The addition of a chip-based FAIMS separation prior to detection by time-of-flight mass spectrometry enables selective transmission of NMP in the presence of cefepime without interference from NMP formed by CID in the mass spectrometer interface. The limits of detection and quantification of NMP in cefepime were 0.011% (w/w) and 0.036% (w/w) NMP in cefepime respectively, well below the 0.3% (w/w) threshold concentration for NMP in cefepime. The % relative standard deviation was 3.9% with linearity for standard additions in the range 0.005 – 0.5 μg/ml NMP.
Novel Aspect (ToC)
FAIMS separation prior to mass spectrometry enables selective transmission of NMP in cefepime without interference from NMP formed by in-source CID.
History
School
- Science
Department
- Chemistry
Published in
Analytical Methods: advancing methods and applicationsVolume
7Pages
34 - 39 (6)Citation
SMITH, R.W. ...et al., 2015. Rapid determination of N-Methylpyrrolidine in Cefepime by combining direct infusion electrospray ionisation-time-of-flight mass spectrometry with field asymmetric waveform ion mobility spectrometry. Analytical Methods, 7, pp. 34-39.Publisher
Royal Society of ChemistryVersion
- AM (Accepted Manuscript)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2015Notes
This paper was accepted for publication in the journal Analytical Methods and the definitive published version is available at: http://dx.doi.org/10.1039/c4ay02026jISSN
1759-9679Publisher version
Language
- en