Shendruk_36375.pdf (3.43 MB)
Rapid dynamics of cell-shape recovery in response to local deformations
journal contribution
posted on 2018-12-10, 13:30 authored by Kristina Haase, Tyler Shendruk, Andrew E. PellingIt is vital that cells respond rapidly to mechanical cues within their microenvironment through changes
in cell shape and volume, which rely upon the mechanical properties of cells’ highly interconnected
cytoskeletal networks and intracellular fluid redistributions. While previous research has largely
investigated deformation mechanics, we now focus on the immediate cell-shape recovery response
following mechanical perturbation by inducing large, local, and reproducible cellular deformations using
AFM. By continuous imaging within the plane of deformation, we characterize the membrane and
cortical response of HeLa cells to unloading, and model the recovery via overdamped viscoelastic
dynamics. Importantly, the majority (90%) of HeLa cells recover their cell shape in o1 s. Despite actin
remodelling on this time scale, we show that cell-shape recovery time is not affected by load duration,
nor magnitude for untreated cells. To further explore this rapid recovery response, we expose cells to
cytoskeletal destabilizers and osmotic shock conditions, which uncovers the interplay between actin and
osmotic pressure. We show that the rapid dynamics of recovery depend crucially on intracellular
pressure, and provide strong evidence that cortical actin is the key regulator in the cell-shape recovery
processes, in both cancerous and non-cancerous epithelial cells
Funding
AEP was supported by the Canada Research Chairs (CRC) program and a Province of Ontario Early Researcher Award. This work was supported by a Natural Sciences and Engineering Research Council (NSERC) Discovery Grant, an NSERC Discovery Accelerator Supplement, a CRC Operating Grant and the Canadian Foundation for Innovation Leaders Opportunity Fund. We acknowledge funding from ERC Advanced Grant 291234 MiCE and EMBO funding ALTF181-2013 to TNS.
History
School
- Science
Department
- Mathematical Sciences
Published in
Soft MatterVolume
13Issue
3Pages
567 - 577Citation
HAASE, K., SHENDRUK, T.N. and PELLING, A.E., 2016. Rapid dynamics of cell-shape recovery in response to local deformations. Soft Matter, 13(3), pp. 567-577.Publisher
© Royal Society of ChemistryVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Acceptance date
2016-11-30Publication date
2016Notes
This paper was submitted for publication to the journal Soft Matter and the definitive published version is available at https://doi.org/10.1039/C6SM02560AISSN
1744-683XeISSN
1744-6848Publisher version
Language
- en