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Regenerative potential of low-concentration SDS-decellularized porcine aortic valved conduits in vivo

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posted on 2019-11-11, 09:55 authored by José Rodolfo Paniagua Gutierrez, Helen Berry, Sotiris KorossisSotiris Korossis, Saeed Mirsadraee, Sergio Veiga Lopes, Francisco da Costa, John Kearney, Kevin Watterson, John Fisher, Eileen Ingham
© 2015 Mary Ann Liebert, Inc. The aims of this study were to determine the functional biocompatibility of low-concentration SDS-decellularized porcine aortic roots in vivo. A previously developed process was modified for 9-And 15-mm-diameter aortic roots to facilitate implantation into the porcine abdominal aorta (n=3) and juvenile sheep right ventricular outflow tract (n=7), respectively. Native allogeneic aortic roots were used as controls. Acellular porcine roots explanted from pigs at weeks were largely repopulated with stromal cells of appropriate phenotype, and there was evidence that macrophages were involved in the regenerative process. Native allogeneic roots were subject to a classic allograft rejection response. Acellular porcine roots explanted from sheep at 6 months showed evidence of appropriate cellular repopulation, again with evidence of a role for macrophages in the regenerative process. There was some degree of calcification of two of the explanted acellular roots, likely due to incomplete removal of DNA before implantation. Native allogeneic ovine roots were subject to a classic allograft rejection response involving T cells, which resulted in overtly calcified and damaged tissues. The study highlighted (1) the importance of removal of DNA from acellular porcine valved roots to avoid calcification and (2) a role for macrophages in the regeneration of low-concentration SDS-decellularized aortic roots, as has been reported for other acellular biological extracellular matrix scaffolds.

Funding

Children‘s Heart Surgery Fund

EPSRC

WELMEC, a Centre of Excellence in Medical Engineering funded by the Wellcome Trust and EPSRC, under grant number WT 088908/Z/09/Z

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Tissue Engineering Part A

Volume

21

Issue

1-2

Pages

332 - 342

Publisher

Mary Ann Liebert Inc

Version

  • VoR (Version of Record)

Rights holder

© the Authors

Publisher statement

This is an Open Access Article. It is published by Mary Ann Liebert under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY NC SA). Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-sa/4.0/

Publication date

2014-09-26

Copyright date

2015

ISSN

1937-3368

eISSN

1937-335X

Language

  • en

Depositor

Prof Sotiris Korossis. Deposit date: 11 November 2019