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Specific two-photon imaging of live cellular and deep-tissue lipid droplets by lipophilic AIEgens at ultra-low concentration

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posted on 17.07.2018 by Guangle Niu, Ruoyao Zhang, John P. Kwong, Jacky W.Y. Lam, Congping Chen, Jianguo Wang, Yuncong Chen, Xing Feng, Ryan T. Kwok, Herman H.-Y. Sung, Ian D. Williams, Mark Elsegood, Jianan Qu, Chao Ma, Kam S. Wong, Xiaoqiang Yu, Ben Zhong Tang
Lipid droplets are highly associated with obesity, diabetes, inflammatory disorders and cancer. A reliable two-photon dye for specific lipid droplets imaging in live cells and live tissues at ultra-low concentration has rarely been reported. In this work, four new aggregation-induced emission luminogens (AIEgens) based on the naphthalene core were designed and synthesized for specific two-photon lipid droplets staining. The new molecules, namely NAP AIEgens, exhibit large Stokes shift (>110 nm), high solid-state fluorescence quantum yield (up to 30%), good two-photon absorption cross section (45–100 GM at 860 nm), high biocompatibility and good photostability. They could specifically stain lipid droplets at ultra-low concentration (50 nM) in a short time of 15 min. Such ultra-low concentration is the lowest value for lipid droplets staining in live cells reported so far. In vitro and ex vivo two-photon imaging of lipid droplets in live cells and live mice liver tissues were successfully demonstrated. In addition, selective visualization of lipid droplets in live mice liver tissues could be achieved at a depth of about 70 μm. These excellent properties render them as promising candidates for investigating lipid droplets-associated physiological and pathological processes in live biological samples.

Funding

This work was partially supported by the National Natural Science Foundation of China (21788102, 51773111, 21490570, and 21490574), the National Basic Research Program of China (973 Program: 2013CB834701 and 2013CB834702), the Research Grants Council of Hong Kong (16301614, 16305015, A-HKUST 605/16, N_HKUST604/14, and AoE/P-02/12), the Innovation and Technology Commission (ITC-CNERC14SC01 and ITCPD/17-9), and the Science and Technology Plan of Shenzhen (JCYJ20170818113851132, JCYJ20170818113840164, JCYJ20160229205601482, and JCYJ20140425170011516).

History

School

  • Science

Department

  • Chemistry

Published in

Chemistry of Materials

Citation

NIU, G. ... et al, 2018. Specific two-photon imaging of live cellular and deep-tissue lipid droplets by lipophilic AIEgens at ultra-low concentration. Chemistry of Materials, 30 (14), pp.4778–4787.

Publisher

© American Chemical Society

Version

AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Acceptance date

02/07/2018

Publication date

2018-07-02

Notes

This document is the Accepted Manuscript version of a Published Work that appeared in final form in Chemistry of Materials, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see https://pubs.acs.org/doi/10.1021/acs.chemmater.8b01943.

ISSN

0897-4756

eISSN

1520-5002

Language

en

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