Loughborough University
Browse
stiffness-of-cell-microenvironment-guides-long-term-cell-growth-in-cell-seeded-collagen-microspheres.pdf (1.31 MB)

Stiffness of cell micro-environment guides long term cell growth in cell seeded collagen microspheres

Download (1.31 MB)
journal contribution
posted on 2018-10-16, 10:26 authored by Maryam Shariatzadeh, C.M. Perrault, D. Lacroix
Mesenchymal stem cells are widely implicated as a cell source for tissue engineering of skeletal tissue in cell-based therapy. Physical and mechanical cues are potent controlling factors in cell differentiation and can be implemented as a guide to study cellular response, matrix production and tissue regeneration. Microspheres were produced by gelation of bovine collagen type I with concentration of 2 mg/mL and 1,000-2,000 cells per droplet. Short and long term cell viability of human embryonic stem cell-derived mesenchymal progenitors (hES-MPs) and MG-63 osteoblastic cells as well as collagen microstructure and contraction were monitored during 28 days post encapsulation (pc). Results indicated that collagen concentration, hence mechanical properties of cell’s extracellular micro-environment are important in cell proliferation and differentiation. Contraction of cell-embedded microspheres was found to be vital in cell adaptation and the remodelling of their new environment. It was also found that collagen concentration of 2 mg/mL supports proliferation of hES-MPs while higher collagen concentration promoted the viability of MG-63s. Results of hES-MPs characterization in 3D soft environment and mechanically stimulated hES-MPs collagen microspheres can be used in cells/therapeutic carriers, implants in bone and cartilage healing applications. The microspheres developed in this study can also be used as a tool to build more optimised construct to transfer mechanically stimulated stem cells to the specific area of a defective bone which would add significant benefit to the field of bone regeneration.

Funding

Financial support from the European Research Council (258321) is acknowledged.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Archives of Clinical and Biomedical Research

Volume

2

Issue

5

Pages

167 - 182

Citation

SHARIATZADEH, M., PERRAULT, C.M. and LACROIX, D., 2018. Stiffness of cell micro-environment guides long term cell growth in cell seeded collagen microspheres. Archives of Clinical and Biomedical Research, 2 (5), pp.167-182.

Publisher

Fortune Journals

Version

  • VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/ by/4.0/

Publication date

2018

Notes

This is an Open Access Article. It is published by Fortune Journals under the Creative Commons Attribution 4.0 International Licence (CC BY). Full details of this licence are available at: https://creativecommons.org/licenses/by/4.0/

ISSN

2572-5017

Language

  • en