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Suspension cultures of bone-marrow-derived mesenchymal stem cells: effects of donor age and glucose level

journal contribution
posted on 26.02.2015 by Alexandra Stolzing, Eva Bauer, Andrew Scutt
Both ageing and diabetes are associated with reduced numbers and functional viability of mesenchymal stem cells (MSCs) in vivo which in turn lead to degenerative pathologies of the musculoskeletal system. The overall aim of this study was to elucidate the effects of age and raised glucose levels on the proliferation and selfrenewal of rat nonadherent bone marrow MSCs (Na-BM-MSCs) in suspension cultures. MSC cultures isolated from 3- and 12-month-old rats were maintained using the "pour-off" method for up to 14 days in media containing different glucose levels and the phenotype, growth characteristics, colony forming unit-fibroblastic (CFU-f) numbers, and pluripotency characteristics of these cells were determined. This study indicates that rat adult bone marrow harbors pluripotent Na-BM-MSCs that seem to be unaffected by ageing during in vitro expansion. The Na-BM-MSCs express the pluripotency markers Oct4, Sox2, and Nanog. It was found that culture in high-glucose- containing medium had a negative effect on colony formation and differentiation. In contrast to classical MSC cultures, the generation of colonies by Na-BM-MSCs in suspension culture was not reduced in the older animals. The Na-BM-MSCs were found to express the pluripotency markers Oct4, Sox2, and Nanog, suggesting a more primitive stage of differentiation as compared with adherent MSCs. These data indicate that rat adult bone marrow harbors a population of pluripotent Na-BM-MSCs that appear to be relatively unaffected by ageing during in vitro expansion in suspension. © 2012 Mary Ann Liebert, Inc.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Stem Cells and Development

Volume

21

Issue

14

Pages

2718 - 2723

Citation

STOLZING, A., BAUER, E. and SCUTT, A., 2012. Suspension cultures of bone-marrow-derived mesenchymal stem cells: effects of donor age and glucose level. Stem Cells and Development, 21 (14), pp. 2718 - 2723

Publisher

© Mary Ann Liebert, Inc.

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2012

Notes

Closed access. Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/scd.2011.0406

ISSN

1547-3287

eISSN

1557-8534

Language

en

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